Application of Biosensors Based on Aptamers and Antibodies in the Detection of Estradiol

doi:10.19894/j.issn.1000-0518.210108

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (3): 374-390.DOI: 10.19894/j.issn.1000-0518.210108

• Review • Previous Articles Next Articles

Application of Biosensors Based on Aptamers and Antibodies in the Detection of Estradiol

Tao WANG¹^,², Sha LIU², Bao-Lin LIU¹(), Zhi-Xian GAO²()

^1.School of Medical Devices and Food，University of Shanghai for Science and Technology，Shanghai 200093，China
^2.Institute of Environmental Medicine and Operational Medicine，Academy of Military Medicine，Academy of Military Sciences，Tianjin Key Laboratory of Environmental and Food Safety Risk Monitoring Technology，Tianjin 300050，China

Received:2021-03-11 Accepted:2021-06-26 Published:2022-03-01 Online:2022-03-15
Contact: Bao-Lin LIU,Zhi-Xian GAO
About author:blliuk@163.com； gaozhx@163.com
Supported by:
the National Natural Science Foundation of China(81773482)

Abstract

Abstract:

Estradiol （E₂） is one of the major environmental estrogens， which has the characteristics of strong action and extensive harmness， and can disrupt the normal endocrine function of human and animal bodies， thus causing adverse effects. Therefore， the rapid and accurate detection of E₂ is of great importance for food safety and public health. Traditional detection methods are mainly based on large-scale instrument analysis. These methods have high sensitivity and accuracy， but also have drawbacks such as tedious operation， high cost and limitation to laboratory. In recent years， researchers have developed a variety of biosensors to detect E₂. In the early stage， there will be problems such as low sensitivity and interference signals. There is a lot of room for improvement. Gradually， a new method with simple operation， high detection sensitivity and rapid on-site detection can be explored. Based on a brief introduction to the characteristics of aptamers and antibodies， this article summarizes the latest research progress in E₂detection of various biosensors using aptamers and antibodies as recognition signals. Finally， this review provides new prospects for its research direction.

Key words: Estradiol detection, Aptamer, Antibody, Biosensor

CLC Number:

O657

Tao WANG, Sha LIU, Bao-Lin LIU, Zhi-Xian GAO. Application of Biosensors Based on Aptamers and Antibodies in the Detection of Estradiol[J]. Chinese Journal of Applied Chemistry, 2022, 39(3): 374-390.

Figures/Tables 10

References 77

1	ZHANG G， LI T， ZHANG J， et al. A simple FRET-based turn-on fluorescent aptasensor for 17β-estradiol determination in environmental water， urine and milk samples［J］. Sens Actuators B， 2018， 273： 1648-1653.
2	马嘉琪，姚晓琳，刘思杰，等. 基于不同探针免疫层析方法对17β-雌二醇的分析灵敏度比较研究［J］. 分析测试学报， 2021， 40（3）： 310-317.
	MA J Q， YAO X L， LIU S J， et al. Comparative study on the sensitivity of 17β-estradiol based on different probe immunochromatographic methods［J］. J Instrum Anal， 2021， 40（3）： 310-317.
3	马丽莎，戴晓欣，谢文平，等. QuEChERS/GC-MS法同时测定鱼、虾中的雌二醇与己烯雌酚残留［J］. 分析测试学报， 2015， 34（1）： 62-66.
	MA L S， DAI X X， XIE W P， et al. Simultaneous determination of diethylstilbestrol and estradiol in fish and shrimp by GC-MS with QuEChERS sample preparation［J］. J Instrum Anal， 2015， 34（1）： 62-66.
4	ZHANG Y， ZHOU J L， NING B. Photodegradation of estrone and 17β-estradiol in water［J］. Water Res， 2007， 41（1）： 19-26.
5	NI X， XIA B， WANG L， et al. Fluorescent aptasensor for 17β-estradiol determination based on gold nanoparticles quenching the fluorescence of rhodamine B［J］. Anal Biochem， 2017， 523： 17-23.
6	王全林，张爱芝，陈立仁. 超高效液相色谱-串联质谱法对奶制品中苯甲酸雌二醇残留的测定［J］. 分析测试学报， 2009， 28（9）： 1069-1073.
	WANG Q L， ZHANG A Z， CHEN L R. Determination of estradiol benzoate residues in dairy products by ultra performance liquid chromatography-tandem mass spectrometry［J］. J Instrum Anal， 2009， 28（9）： 1069-1073.
7	HINTEMAN T， SCHNEIDER C， SCHOLER H F， et al. Field study using two immunoassays for the determination of estradiol and ethinylestradiol in the aquatic environment［J］. Water Res， 2006， 40（12）： 2287-94.
8	HAN D H， DENISON M S， TACHIBANA H， et al. Relationship between estrogen receptor-binding and estrogenic activities of environmental estrogens and suppression by flavonoids［J］. Biosci Biotechnol Biochem， 2002， 66（7）： 1479-87.
9	GANMAA D， WANG P Y， QIN L Q， et al. Is milk responsible for male reproductive disorders？［J］. Med Hypotheses， 2001， 57（4）： 510-514.
10	PAPE-ZAMBITOD A， ROBERTS R F， KENSINGER R S. Estrone and 17β-estradiol concentrations in pasteurized-homogenized milk and commercial dairy products［J］. J Dairy Sci， 2010， 93（6）： 2533-2540.
11	JURGENS M D， HOLTHAUS K I E， JOHNSON A C， et al. The potential for estradiol and ethinylestradiol degradation in English rivers［J］. Environ Toxicol Chem， 2002， 21（3）： 480-488.
12	QI X， HU H， YANG Y， et al. Graphite nanoparticle as nanoquencher for 17β-estradiol detection using shortened aptamer sequence ［J］. Analyst， 2018， 143（17）： 4163-4170.
13	MASIKINI M， GHICA M E， BAKER P G L， et al. Electrochemical sensor based on multi-walled carbon nanotube/gold nanoparticle modified glassy carbon electrode for detection of estradiol in environmental samples［J］. Electroanalysis， 2019， 31（10）： 1925-1933.
14	LU H， XU S. Mesoporous structured estrone imprinted Fe₃O₄@SiO₂@mSiO₂ for highly sensitive and selective detection of estrogens from water samples by HPLC［J］. Talanta， 2015， 144： 303-311.
15	FARRE M， KUSTER M， BRIX R， et al. Comparative study of an estradiol enzyme-linked immunosorbent assay kit， liquid chromatography-tandem mass spectrometry， and ultra performance liquid chromatography-quadrupole time of flight mass spectrometry for part-per-trillion analysis of estrogens in water samples［J］. J Chromatogr A， 2007， 1160（1/2）： 166-175.
16	CHOI M H， KIM K R， CHUNG B C. Determination of estrone and 17β-estradiol in human hair by gas chromatography-mass spectrometry［J］. Analyst， 2000， 125（4）： 711-714.
17	SEIFERT M， HAINDL S， HOCK B. Development of an enzyme linked receptor assay （ELRA） for estrogens and xenoestrogens［J］. Anal Chim Acta， 1999， 386（3）： 191-199.
18	NORTH S H， LOCK E H， TAITT C R， et al. Critical aspects of biointerface design and their impact on biosensor development［J］. Anal Bioanal Chem， 2010， 397（3）： 925-933.
19	付志强，熊艳. 便携式生物光学传感器的研究进展［J］. 生物技术通报， 2021， 37（3）： 219-226.
	FU Z Q， XIONG Y. Research progress of portable bio-optical sensors［J］. Biotechnol Bull， 2021， 37（3）： 219-226.
20	NUNES DA SILVA D， LEIJOTO DE OLIVEIRA H， BORGES K B， et al. Sensitive determination of 17β-estradiol using a magneto sensor based on magnetic molecularly imprinted polymer［J］. Electroanalysis， 2020， 33（2）： 506-514.
21	GUGOASA L A， STEFAN-VAN STADEN R I， CALENIC B， et al. Multimode sensors as new tools for molecular recognition of testosterone， dihydrotestosterone and estradiol in children's saliva［J］. J Mol Recognit， 2015， 28（1）： 10-19.
22	WANG X， PEI K， SUN H， et al. A magnetic relaxation switch sensor for determination of 17β-Estradiol in milk and eggs based on aptamer-functionalized Fe₃O₄@Au nanoparticles［J/OL］. J Sci Food Agric， 2021， 101（13）： 5697-5706.
23	PRASKOVIYA B， SVITLANA S， LUC V， et al. Self-tuning interfacial architecture for estradiol detection by surface plasmon resonance biosensor［J］. Biosens Bioelectron， 2017， 90： 91-95.
24	BRODY EN， GOLD L. Aptamers as therapeutic and diagnostic agents［J］. J Biotechnol， 2000， 74（1）： 5-13.
25	孙娜娜. 基于啶虫脒适配体和抗体的上转换荧光快速检测方法研究［D］. 南京：南京农业大学， 2018.
	SUN N N. Study on the up-conversion fluorescence rapid detection method based on acetamiprid aptamer and antibody［D］. Nanjing： Nanjing Agricultural University， 2018.
26	WATTS H J， LOWE C R， POLLARD-KNIGHT D V. Optical biosensor for monitoring microbial cells［J］. Anal Chem， 1994， 66（15）： 2465-2470.
27	SANTOS A， BALDERRAMA V S， ALBA M， et al. Nanoporous anodic alumina barcodes： toward smart optical biosensors［J］. Adv Mater， 2012， 24（8）： 1050-1054.
28	徐林. 基于信号放大的荧光生物传感器用于生物小分子的高灵敏检测［D］. 重庆：重庆理工大学， 2020.
	XU L. Fluorescence biosensor based on signal amplification is used for highly sensitive detection of small biological molecules［D］. Chongqing： Chongqing University of Technology， 2020.
29	BAHREYNI A， LUO H， RAMEZANI M， et al. A fluorescent sensing strategy for ultrasensitive detection of oxytetracycline in milk based on aptamer-magnetic bead conjugate， complementary strand of aptamer and PicoGreen［J］. Spectrochim Acta A， 2021， 246： 119009.
30	董亚非，胡文晓，钱梦瑶，等. 基于DNA适应体的荧光生物传感器［J］. 电子与信息学报， 2020， 42（6）： 75-83.
	DONG Y F， HU W X， QIAN M Y， et al. Fluorescence biosensor based on DNA adaptor［J］. J Electr Inf Technol， 2020， 42（6）： 75-83.
31	HU L， CHENG Q， CHEN D， et al. Liquid-phase exfoliated graphene as highly-sensitive sensor for simultaneous determination of endocrine disruptors： diethylstilbestrol and estradiol［J］. J Hazard Mater， 2015， 283： 157-163.
32	WANG Y， ZHAO X， ZHANG M， et al. A fluorescent amplification strategy for high-sensitive detection of 17β-estradiol based on EXPAR and HCR［J］. Anal Chim Acta， 2020， 1116： 1-8.
33	HUANG H， SHI S， GAO X， et al. A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine， dopamine and 17β-estradiol detection［J］. Biosens Bioelectron， 2016， 79： 198-204.
34	JAZAYERI M H， AGHAIE T， AVAN A， et al. Colorimetric detection based on gold nano particles （GNPs）： an easy， fast， inexpensive， low-cost and short time method in detection of analytes （protein， DNA， and ion）［J］. Sensing Bio-Sensing Res， 2018， 20： 1-8.
35	王燕，周化岚，施沁怡，等. 基于金纳米粒子光学性质的比色传感器及其在食品安全检测中的应用［J］. 理化检验（化学分册）， 2019， 55（12）： 1476-1482.
	WANG Y， ZHOU H L， SHI Q Y， et al. Colorimetric sensor based on optical properties of gold nanoparticles and its application in food safety detection［J］. Phys Chem Ins （Chem Sect）， 2019， 55（12）： 1476-1482.
36	JALALIAN S H， LAVAEE P， RAMEZANI M， et al. An optical aptasensor for aflatoxin M1 detection based on target-induced protection of gold nanoparticles against salt-induced aggregation and silica nanoparticles［J］. Spectrochim Acta A， 2021， 246.
37	王欣. 基于核酸适配体的牛奶和鸡蛋中雌二醇纳米金比色检测［J］. 农业机械学报， 2019， 2020， 51（9）： 319-328.
	WANG X. Nucleic acid aptamer-based colorimetric detection of estradiol nanogold in milk and eggs［J］. Trans Chinese Soc Agric Mach， 2020， 51（9）： 319-328.
38	LI Y， XU J， JIA M， et al. Colorimetric determination of 17β-estradiol based on the specific recognition of aptamer and the salt-induced aggregation of gold nanoparticles［J］. Mater Lett， 2015， 159： 221-224.
39	ZHANG D， ZHANG W， YE J， et al. A Label-free colorimetric biosensor for 17β-estradiol detection using nanoparticles assembled by aptamer and cationic polymer［J］. Aust J Chem， 2016， 69（1）： 12-19.
40	黄磊，王欣，刘厦，等. 基于适配体的生物传感器在双酚A检测中的应用［J］. 分析化学， 2021， 49（2）： 172-183.
	HUANG L， WANG X， LIU S， et al. Application of aptamer-based biosensor in the detection of bisphenol A［J］. Chinese J Anal Chem， 2021， 49（2）： 172-183.
41	王海旺. 基于目标物介导SERS金纳米颗粒自组装的新型生物传感器研究［D］. 济南大学， 2019.
	WANG H W. Research on a novel biosensor based on target mediated self-assembly of SERS gold nanoparticles［D］. Universty of Jinan， 2019.
42	GANDRA N， ABBAS A， TIAN L， et al. Plasmonic planet-satellite analogues： hierarchical self-assembly of gold nanostructures［J］. Nano Lett， 2012， 12（5）： 2645-2651.
43	SIYAO L， YUQING C， YING W， et al. Group-targeting detection of total steroid estrogen using surface-enhanced raman spectroscopy［J］. Anal Chem， 2019， 91（12）： 7639-7647.
44	谢晓慧. 适配体修饰核壳纳米材料表面增强拉曼光谱检测17β-雌二醇方法研究［D］. 华南理工大学， 2018.
	XIE X H. Aptamer-modified core-shell nanomaterials surface enhanced Raman spectroscopy method for the detection of 17β-estradiol［D］. South China University of Technology， 2018.
45	姚武，崔朋，胡晓倩. 基于信号增强的电化学发光适配体传感器检测三磷酸腺苷［J］. 发光学报， 2020， 41（6）： 117-125.
	YAO W， CUI P， HU X Q. Electrochemiluminescence aptamer sensor based on signal enhancement for the detection of adenosine triphosphate［J］.Chinese J Lumin， 2020， 41（6）： 117-125.
46	ZHAO G， WANG Y， LI X， et al. Dual-Quenching electrochemiluminescence strategy based on three-dimensional metal-organic frameworks for ultrasensitive detection of amyloid-beta［J］. Anal Chem， 2019， 91（3）： 1989-1996.
47	XU Y， LIU J， GAO C， et al. Applications of carbon quantum dots in electrochemiluminescence： a mini review［J］. Electrochem Commun， 2014， 48： 151-154.
48	KIRSCHBAUM S E， BAEUMNER A J. A review of electrochemiluminescence （ECL） in and for microfluidic analytical devices ［J］. Anal Bioanal Chem， 2015， 407（14）： 3911-3926.
49	ZHANG J J， CAO J T， SHI G F， et al. Label-free and sensitive electrochemiluminescence aptasensor for the determination of 17β-estradiol based on a competitive assay with cDNA amplification［J］. Anal Methods， 2014， 6（17）： 6796-6801.
50	LIU Y， LI B， YAO Y， et al. An electrochemiluminescence sensor for 17β-estradiol detection based on resonance energy transfer in alpha-FeOOH@CdS/Ag NCs［J］. Talanta， 2021， 221： 121479.
51	邓昆. 非标记型电化学适配体传感器的构建及其在蛋白质检测中的应用［D］. 第三军医大学， 2013.
	DENG K. Construction of a non-labeled electrochemical aptamer sensor and its application in protein detection［D］. Third Military Medical University， 2013.
52	李凤琴，俞志刚，韩贤达，等. 检测牛奶和水中卡那霉素残留物的电化学适配体传感器研究进展［J］. 分析科学学报， 2019， 35（4）： 514-520.
	LI F Q， YU Z G， HAN X D， et al. Research progress of electrochemical aptamer sensors for detecting kanamycin residues in milk and water［J］. J Anal Sci， 2019， 35（4）： 514-520.
53	ZHAO L， YANG K， HUANG W， et al. Electrochemical biosensors based on two dimensional nano-materials for disease diagnosis［J］. Chinese Sci Bull， 2016， 61（11）： 1222-1232.
54	LIN X， NI Y， KOKOT S. Electrochemical cholesterol sensor based on cholesterol oxidase and MoS₂-AuNPs modified glassy carbon electrode［J］. Sens Actuators B： Chem， 2016， 233： 100-106.
55	贺彩梅，郑景伊，李晓霞. 基于二硫化钼/纳米金和硫堇/纳米信号放大的雌二醇电化学适配体传感器［J］. 高等学校化学学报， 2019， 40（10）： 2090-2096.
	HE C M， ZHENG J Y， LI X X. Estradiol electrochemical aptamer sensor based on molybdenum disulfide/nano gold and thionine/nano signal amplification［J］. Chem J Chinese Univ， 2019， 40（10）： 2090-2096.
56	LIU M， KE H， SUN C， et al. A simple and highly selective electrochemical label-free aptasensor of 17β-estradiol based on signal amplification of bi-functional graphene［J］. Talanta， 2019， 194： 266-272.
57	MATTEA CARMEN C， PAOLA B， JACOPO C， et al. Electrospray deposition as a smart technique for laccase immobilisation on carbon black-nanomodified screen-printed electrodes［J］. Biosens Bioelectron， 2020， 163： 112299.
58	蓝庆春. 新型无标记电化学免疫传感器的构建及其在肿瘤标志物检测中的应用［D］. 扬州大学， 2020.
	LAN Q C. Construction of a new label-free electrochemical immunosensor and its application in the detection of tumor markers［D］. Yangzhou University， 2020.
59	聂荣彬. 新型化学发光免疫传感器的构建与应用［D］. 东北师范大学， 2020.
	NIE R B. Construction and application of a new type of chemiluminescence immunosensor［D］. Northeast Normal University， 2020.
60	郝一雄，郭金虎. 纳米材料在生物免疫传感器中应用的研究进展［J］. 同济大学学报（医学版）， 2020， 41（4）： 131-137.
	HAO Y X，GUO J H. Research progress in the application of nanomaterials in biological immunosensors［J］. J Tongji Univ Med Sci， 2020， 41（4）： 131-137.
61	孙艳，孙锋，杨玉孝，等. 光学免疫传感器技术与应用［J］. 仪表技术与传感器， 2002 （7）： 5-8.
	SYN Y， SUN F， YANG Y X， et al. Optical immunosensor technology and application［J］. Instrum Technol Sens， 2002（7）： 5-8.
62	KIM H R， KIM J H， KIM T S， et al. Optical detection of deoxyribonucleic acid hybridization using an anchoring transition of liquid crystal alignment［J］. Appl Phys Lett， 2005， 87（14）： 105.
63	单迪迪，文晓刚，刘兰华，等. 基于阵列倏逝波荧光传感器的雌二醇免疫检测方法［J］. 光谱学与光谱分析， 2018， 38（10）： 3148-3251.
	SHAN D D， WEN X G， LIU L H， et al. Estradiol immunodetection method based on array evanescent wave fluorescence sensor［J］.Spectrosc Spectr Anal， 2018， 38（10）： 3148-3251.
64	SCALA-BENUZZI M L， TAKARA E A， ALDERETE M， et al. Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor［J］. Microchem J， 2018， 141： 287-293.
65	FUNARI R， DELLA VENTURA B， ALTUCCI C， et al. Single molecule characterization of UV-activated antibodies on gold by atomic force microscopy［J］. Langmuir， 2016， 32（32）： 8084-8091.
66	MINOPOLI A， SAKAČ N， LENYK B， et al. LSPR-based colorimetric immunosensor for rapid and sensitive 17β-estradiol detection in tap water［J］. Sens Actuators B： Chem， 2020， 308： 127699.
67	刘小红，邓华，常林，等. 环境雌激素SERS检测的研究进展［J］. 光谱学与光谱分析， 2020， 40（10）： 3038-3047.
	LIU X H， DENG H， CHANG L， et al. Research progress of environmental estrogen SERS detection［J］. Spectrosc Spectr Anal，2020， 40（10）： 3038-3047.
68	WANG R， CHON H， LEE S， et al. Highly sensitive detection of hormone estradiol E₂ using surface-enhanced raman scattering based immunoassays for the clinical diagnosis of precocious puberty［J］. ACS Appl Mater Interfaces， 2016， 8（17）： 10665-72.
69	李如男，郭兴道，陈文杨，等. 女性关键生育力评价指标雌二醇、抗苗勒管激素及窦卵泡数目的拉曼光谱检测［J］. 第二军医大学学报， 2019， 363（11）： 61-68.
	LI R N， GUO X D， CHEN W Y， et al. Raman spectroscopy detection of estradiol， anti-Müllerian hormone and the number of antral follicles， the key indicators of female fertility evaluation［J］. J Second Mil Med Univ， 2019， 363（11）： 61-68.
70	HE S， XIE W， ZHANG W， et al. Multivariate qualitative analysis of banned additives in food safety using surface enhanced Raman scattering spectroscopy［J］. Spectrochim Acta A： Mol Biomol Spectrosc， 2015， 137： 1092-1099.
71	白家磊. 典型激素类污染物检测的双通道ICA与ECL-RET传感技术研究［D］. 中国人民解放军军事医学科学院， 2016.
	BAI J L. Research on dual-channel ICA and ECL-RET sensing technology for the detection of typical hormone pollutants［D］. Chin Acad Mil Med Sci， 2016.
72	胡梅. 抗球虫药单克隆抗体的制备及其在电化学免疫传感器中的应用［D］. 江南大学， 2019.
	HU M. Preparation of anticoccidial monoclonal antibody and its application in electrochemical immunosensor［D］. Jiangnan University， 2019.
73	ZHANG X， LI Y， LV H， et al. Sandwich-type electrochemical immunosensor based on Au@Ag supported on functionalized phenolic resin microporous carbon spheres for ultrasensitive analysis of alpha-fetoprotein［J］. Biosens Bioelectron， 2018， 106： 142-148.
74	ZHANG Y， LI J， WANG Z， et al. Label-free electrochemical immunosensor based on enhanced signal amplification between Au@Pd and CoFe₂O₄/graphene nanohybrid［J］. Sci Rep， 2016， 6： 23391.
75	SEN Z， BIN D， HE L， et al. Metal ions-based immunosensor for simultaneous determination of estradiol and diethylstilbestrol［J］. Biosens Bioelectron， 2014， 52： 225-231.
76	YAN T， WU T， WEI S， et al. Photoelectrochemical competitive immunosensor for 17β-estradiol detection based on ZnIn₂S₄@NH₂-MIL-125（Ti） amplified by PDA NS/Mn：ZnCdS［J］. Biosens Bioelectron， 2020， 148：111739.
77	LI R X， LIU Y X， YAN T， et al. A competitive photoelectrochemical assay for estradiol based on in situ generated CdS-enhanced TiO₂［J］. Biosens Bioelectron， 2015， 66： 596-602.

传感器类型 Sensors type	检测体系 Detection system	线性范围 Linear range	检出限 Limit of detection	实际样品 Real sample	参考文献 References
基于荧光的生物传感器 Biosensor based on fluorescence	FAM-BHQ1	0.1~10 μg/mL	0.1 ng/mL	Milk	［1］
	AuNPs/Rho B	0.48~200 nmol/L	0.48 nmol/L	Water	［5］
	FAM/GN	0~20 ng/mL	1.02 ng/mL	Water	［12］
	EXPAR-HCR	0.4~800 pg/mL	0.37 pg/mL	Milk， Water	［32］
	Ru-QDs	0.08~0.4 μmol/L	37 nmol/L	FBS	［33］
基于金纳米比色的生物传感器Biosensor based on gold nanometer colorimetry	AuNPs	0.5～0.8 nmol/L 0.3～0.9 nmol/L	0.183 nmol/L 0.185 nmol/L	Milk Egg	［37］
基于金纳米比色的生物传感器Biosensor based on gold nanometer colorimetry	PDDA/AuNPs	1.57~350 nmol/L	1.57 nmol/L	Water	［39］
基于表面增强拉曼散射的生物传感器Biosensor based on surface?enhanced raman scattering	Au@Ag CS NPs-MBA	0.01~50 nmol/L	5 pmol/L	Water	［43］
	Au@Ag CS NPs-Cy3	1×10^-13 ~ 1×10^-9 mol/L	0.748 pg/L	Water	［44］
基于电化学发光的生物传感器Biosensor based on electrochemiluminescence	Ru（bpy）₃²⁺	0.01~10 nmol/L	1.1×10^-12 mol/L	Serum， Tap water	［49］
基于电化学发光的生物传感器Biosensor based on electrochemiluminescence	α-FeOOH@CdS-AgNCs-DNA	0.01~10 pg/mL	0.003 pg/mL	Serum	［50］
基于电化学的生物传感器 Electrochemical?based biosensor	MoS₂/GNPs-GNPs/THI	1.0×10^-14 ~ 5.0×10^-12 mol/L	4.2×10^-15 mol/L	Water	［55］
	Apt-G/MCH-Au/ DNase I	0.019~2.7 ng/L	0.014 ng/L	Water	［56］
	CDs/SPCE	1.0×10^-7 ~ 1.0×10^-12 mol/L	0.5×10^-12 mol/L	Water	［57］

传感器类型 Sensors type	检测体系 Detection system	线性范围 Linear range	检出限 Limit of detection	实际样品 Real sample	参考文献 References
基于荧光的生物传感器 Biosensor based on fluorescence	FAM-BHQ1	0.1~10 μg/mL	0.1 ng/mL	Milk	［1］
	AuNPs/Rho B	0.48~200 nmol/L	0.48 nmol/L	Water	［5］
	FAM/GN	0~20 ng/mL	1.02 ng/mL	Water	［12］
	EXPAR-HCR	0.4~800 pg/mL	0.37 pg/mL	Milk， Water	［32］
	Ru-QDs	0.08~0.4 μmol/L	37 nmol/L	FBS	［33］
基于金纳米比色的生物传感器Biosensor based on gold nanometer colorimetry	AuNPs	0.5～0.8 nmol/L 0.3～0.9 nmol/L	0.183 nmol/L 0.185 nmol/L	Milk Egg	［37］
基于金纳米比色的生物传感器Biosensor based on gold nanometer colorimetry	PDDA/AuNPs	1.57~350 nmol/L	1.57 nmol/L	Water	［39］
基于表面增强拉曼散射的生物传感器Biosensor based on surface?enhanced raman scattering	Au@Ag CS NPs-MBA	0.01~50 nmol/L	5 pmol/L	Water	［43］
	Au@Ag CS NPs-Cy3	1×10^-13 ~ 1×10^-9 mol/L	0.748 pg/L	Water	［44］
基于电化学发光的生物传感器Biosensor based on electrochemiluminescence	Ru（bpy）₃²⁺	0.01~10 nmol/L	1.1×10^-12 mol/L	Serum， Tap water	［49］
基于电化学发光的生物传感器Biosensor based on electrochemiluminescence	α-FeOOH@CdS-AgNCs-DNA	0.01~10 pg/mL	0.003 pg/mL	Serum	［50］
基于电化学的生物传感器 Electrochemical?based biosensor	MoS₂/GNPs-GNPs/THI	1.0×10^-14 ~ 5.0×10^-12 mol/L	4.2×10^-15 mol/L	Water	［55］
	Apt-G/MCH-Au/ DNase I	0.019~2.7 ng/L	0.014 ng/L	Water	［56］
	CDs/SPCE	1.0×10^-7 ~ 1.0×10^-12 mol/L	0.5×10^-12 mol/L	Water	［57］

传感器类型 Sensors type	检测体系 Detection system	线性范围 Linear range	检出限 Limit of detection	实际样品 Real sample	参考文献 References
基于荧光的生物传感器 Biosensor based on fluorescence	平面波导型倏逝波 Paper microzone-PVA+SBA	0.08~2.52 μg/L 0~100 ng/L	0.05 μg/L 0.05 ng/L	Water Water	［63］［64］
基于金纳米比色的生物传感器 Biosensor based on gold nanometer colorimetry	AuNPs	3~10⁵ pg/mL	3 pg/mL	Water	［66］
基于表面增强拉曼散射的生物传感器 Biosensor based on surface-enhanced raman scattering	SERS nano-tags	0.1~1000 pg/mL	0.65 pg/mL	Serum	［68］
基于电化学发光的生物传感器 Biosensor based on electrochemiluminescence	Fe₃O₄@Au-CdS QD	2.0×10^-10～ 2.0×10^-4 mg/mL	3.2×10^-12 mg/mL	Veterinary drug residues	［71］
基于电化学的生物传感器 Biosensor based on electrochemical	Au@Pd-CoFe₂O₄/rGO	0.01~18.0 ng/mL	3.3 pg/mL	Water	［74］
基于电化学的生物传感器 Biosensor based on electrochemical	Fe₃O₄-NH₂/SWASV/EIS	0.05~100000 pg/mL	0.015 pg/mL	Water	［75］
基于光电化学的生物传感器 Biosensor based on photoelectrochemistry	ZnIn₂S₄@NH₂-MIL-125（Ti）	0.0005~20 ng/mL	0.3 pg/mL	Water	［76］
基于光电化学的生物传感器 Biosensor based on photoelectrochemistry	TiO₂-CdS	5~4000 pg/mL	2 pg/mL	Water	［77］

传感器类型 Sensors type	检测体系 Detection system	线性范围 Linear range	检出限 Limit of detection	实际样品 Real sample	参考文献 References
基于荧光的生物传感器 Biosensor based on fluorescence	平面波导型倏逝波 Paper microzone-PVA+SBA	0.08~2.52 μg/L 0~100 ng/L	0.05 μg/L 0.05 ng/L	Water Water	［63］［64］
基于金纳米比色的生物传感器 Biosensor based on gold nanometer colorimetry	AuNPs	3~10⁵ pg/mL	3 pg/mL	Water	［66］
基于表面增强拉曼散射的生物传感器 Biosensor based on surface-enhanced raman scattering	SERS nano-tags	0.1~1000 pg/mL	0.65 pg/mL	Serum	［68］
基于电化学发光的生物传感器 Biosensor based on electrochemiluminescence	Fe₃O₄@Au-CdS QD	2.0×10^-10～ 2.0×10^-4 mg/mL	3.2×10^-12 mg/mL	Veterinary drug residues	［71］
基于电化学的生物传感器 Biosensor based on electrochemical	Au@Pd-CoFe₂O₄/rGO	0.01~18.0 ng/mL	3.3 pg/mL	Water	［74］
基于电化学的生物传感器 Biosensor based on electrochemical	Fe₃O₄-NH₂/SWASV/EIS	0.05~100000 pg/mL	0.015 pg/mL	Water	［75］
基于光电化学的生物传感器 Biosensor based on photoelectrochemistry	ZnIn₂S₄@NH₂-MIL-125（Ti）	0.0005~20 ng/mL	0.3 pg/mL	Water	［76］
基于光电化学的生物传感器 Biosensor based on photoelectrochemistry	TiO₂-CdS	5~4000 pg/mL	2 pg/mL	Water	［77］

[1]	Hong-Zhen PENG, Yu ZHANG, Lin-Jie GUO, Wei SONG, Qing-Nuan LI, Xiang-Ying MENG. Facile One⁃Step Synthesis of WS₂@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application [J]. Chinese Journal of Applied Chemistry, 2022, 39(3): 480-488.
[2]	ZHANG Ya-Jing, WANG Xiao-Hui, XU Ya-Juan, LIU Wen-Shu, ZHAO Li-Hui. Preparation of Graphene Nano-Drug Carrier System and Its Killing Effect on Tumor Cells [J]. Chinese Journal of Applied Chemistry, 2021, 38(6): 693-703.
[3]	ZHANG Yuhui, XING Ligang, CHENG Jiawei, YANG Jidong. Chiral Analysis of Propranolol and Its Aptamer System by Resonance Rayleigh Scattering Spectral Method [J]. Chinese Journal of Applied Chemistry, 2020, 37(3): 359-366.
[4]	HUANG Xuewen, XU Sheng, ZHAO Wei, WEI Wei, LI Xiaojie, LIU Xiaoya. Hydrogen Peroxide Sensor Based on a Polymeric Self-assembled Nanoparticles-Modified Screen-Printed Electrode [J]. Chinese Journal of Applied Chemistry, 2020, 37(2): 235-241.
[5]	MO Yanhong, LI Hui, WANG Bin, XU Xiaohui, LIU Sisi, ZENG Dongdong. Research on the Enhanced Activity of Hemin/G-Quadruplex DNAzyme and Its Application in Biosensors [J]. Chinese Journal of Applied Chemistry, 2020, 37(11): 1249-1261.
[6]	GU Xinyu, LANG Le, WANG Jianwei, ZHAO Lihui. Determination of Aflatoxin B₁ in Food by LC-MS/MS Method Based on Magnetic Bead-Aptamer [J]. Chinese Journal of Applied Chemistry, 2020, 37(11): 1324-1332.
[7]	WEI Guobing,KONG Derong,XU Huihui,YIN Zhaojiang,ZHANG Jing,CUI Hanfeng,HONG Nian,YANG Jie,XIONG Wei,LIU Wenming,GUO Qianwen,CHENG Lin,FAN Hao. A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction [J]. Chinese Journal of Applied Chemistry, 2019, 36(5): 595-602.
[8]	QIN Xiaoli,WANG Minghan,DONG Yifan,SHAO Yuanhua. Electrochemiluminescent Biosensing and Its Application in Rapid Detection of Acute Myocardial Infarction Markers [J]. Chinese Journal of Applied Chemistry, 2018, 35(9): 1107-1112.
[9]	HUANG Zike, LIU Chao, FU Qiangqiang, LI Jin, ZOU Jianmei, XIE Sitao, QIU Liping. Aptamer-based Fluorescence Probe for Bioanalysis and Bioimaging [J]. Chinese Journal of Applied Chemistry, 2018, 35(1): 28-39.
[10]	YANG Shaoming, CHEN Yansheng, LI Ruiqin, LONG Qiyang, DING Suyou. One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition [J]. Chinese Journal of Applied Chemistry, 2015, 32(7): 849-854.
[11]	LI Huiyan, BAI Xiaojing, WANG Fenghong, LI Jing, TANG Jilin. Microcantilever Biosensor for Detection of Adenosine Triphosphate [J]. Chinese Journal of Applied Chemistry, 2015, 32(3): 362-366.
[12]	YANG Shaoming*, ZHA Wenling, SUN Qin, LI Hong, LI Ruiqin, SHANG Peiling. Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin [J]. Chinese Journal of Applied Chemistry, 2014, 31(06): 742-748.
[13]	QU Fengjin, CHEN Fang, HOU Xiuzhang, MA Xiaoyan*. Research Progress in the Sensor Application of Ferrocene and Its Derivatives [J]. Chinese Journal of Applied Chemistry, 2013, 30(12): 1393-1398.
[14]	YANG Shaoming*, ZHA Wenling, LI Hong, SUN Qing, LIU Bin, ZHENG Longzhen. Label-free Aptamer Biosensor Based on Cysteine Blocker as the Electrochemical Redox Probe [J]. Chinese Journal of Applied Chemistry, 2013, 30(05): 549-554.
[15]	AN Lingling1, CHANG Yingcui1, DU Jiangyan1,2,3*. A Novel Hydrogen Peroxide Biosensor Based on the Immobilization of Catalase on Three-Dimensionally Ordered Macroporous Graphene-doped Titanium Dioxide Film [J]. Chinese Journal of Applied Chemistry, 2013, 30(02): 171-177.

Application of Biosensors Based on Aptamers and Antibodies in the Detection of Estradiol

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 77

Related Articles 15

Recommended Articles

Metrics

Comments