Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (3): 359-366.DOI: 10.11944/j.issn.1000-0518.2020.03.190252

• Full Papers • Previous Articles    

Chiral Analysis of Propranolol and Its Aptamer System by Resonance Rayleigh Scattering Spectral Method

ZHANG Yuhuia,XING Ligangb,CHENG Jiaweia,YANG Jidonga*()   

  1. aSchool of Environmental and Chemical Engineering,Chongqing Three Gorges University,Wanzhou,Chongqing 404000,China
    bSchool of Life Science and Technology,Yangtze Normal University;Fuling,Chongqing 408000,China
  • Received:2019-09-23 Accepted:2020-01-16 Published:2020-03-01 Online:2020-03-10
  • Contact: YANG Jidong
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21175015, No.21475014)


This paper presents a new method for chiral identification and analysis of propranolol. The method quoted the systematic evolution of ligands by exponential enrichment technique based on graphene oxide (GO-SELEX). The specific aptamer with high affinity to the cardiovascular drug propranolol was selected after ten rounds of optimization and screening. Then resonance Rayleigh scattering spectroscopy (RRS) was used to detect the specificity of the reaction system. The experiment results showed that S-propranolol and R-propranolol had utterly spectral differences. The RRS spectrum of S-propranolol combined with the specific aptamer was significantly enhanced, while the RRS spectrum of R-propranolol's was almost unchanged. Therefore, the chiral propranolol could be effectively recognized. Based on the investigation of the reaction system and experimental conditions, S-propranolol could be tested experimentally and R-propranolol in the racemate can be calculated. The linear range of S-propranolol was 5~275 nmol/L and the detection limit was 0.5 nmol/L. The method was applied to the determination of racemic tablets with satisfactory results. The RRS spectra of chiral targets system binded with specific aptamer could reveal chirality differences and the chiral recognition of chiral enantiomers can be carried out.

Key words: S-/R-propranolol, aptamer, resonance Rayleigh scattering, chiral analysis