Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (4): 481-488.DOI: 10.11944/j.issn.1000-0518.2015.04.140281

• Full Papers • Previous Articles    

Resonance Rayleigh Scattering, Frequency Doubling Scattering and Fluorescence Spectra of the Eosin Y-Mebendazole System and Their Analytical Applications

LI Qina, *, TIAN Fenglingb, ZHOU Shangc, FU Linglia, LIU Qua, YANG Jidongd   

  1. aChongqing Medical and Health School,Chongqing 408000,China
     bSchool of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China
     cSchool of Chemistry and Chemical Engineering,Yangtze Normal University,Chongqing 408000,China
     dSchool of Chemical and Environmental Engineering,Three Gorges University,Chongqing 404100,China
  • Received:2014-08-11 Online:2015-04-10 Published:2015-04-10
  • Contact: Corresponding author:LI Qin, lecturer; Tel:023-72266041; Fax:023-72267446; E-mail:liqinthebest@163.com; Research interests:molecular spectroscopy analysis
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.21175015), Chongqing Fuling District Science and Technology Commission(FLKJ,No.2013ABB2088)

Abstract:

In pH 3.4~3.9 Britton-Robinson(BR) buffer solution, mebendazole(MBZ) reacts with eosin Y(EY) to form a 1:1 ion-association complex, which not only results in the quenching of fluorescence, but also results in the great enhancement of resonance Rayleigh scattering(RRS) and frequency doubling scattering(FDS). Furthermore, a new RRS spectrum appears, and the maximum RRS wavelength is located at 326 nm. The detection limit for MBZ is 32.31 μg/L using the fluorophotometry, 7.24 μg/L by the RRS method and 11.65 μg/L by the FDS method. Among them, the RRS method has the highest sensitivity. The experimental conditions were optimized and effects of coexisting substances were evaluated. These methods have been applied to the determination of MBZ in tablets and human urine samples. The composition and structure of the ion-association complex and the reaction mechanism are discussed.

 

Key words: resonance rayleigh scattering, frequency doubling scattering, fluorescence quenching, mebendazole, Eosin Y

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