Chinese Journal of Applied Chemistry

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Fluorescence Quenching Reaction of Ceftriaxone with Acriflavine and Its Analytical Application

FU Shenghui1,2, LIU Jian3, LIU Shaopu1*   

  1. (1.School of Chemistry and Chemical Engineering,Southwest China University,Chongqing  400715,China;
    2.Chongqing Banan District Environmental Monitor Station,Chongqing  401320,China;
    3.Chongqing Jiulongpo District Environmental Monitor Station,Chongqing  400050,China)
  • Received:2013-09-22 Revised:2013-11-07 Published:2014-07-10 Online:2014-07-10

Abstract: In pH 2.09~5.02 Britton-Robinson(BR) buffer medium, ceftriaxone(CTRX) reacts with acriflavine(AF) to form an 1∶1 ion association complex, which results in quench of the fluorescence of acriflavine. The measurement wavelength of fluorescence quenching is located at λex/λem=266 nm/506 nm. The linear range for CTRX is from 0.056 mg/L to 5.0 mg/L, and its detection limit is 0.016 mg/L. The fluorescence spectra characteristics, the optimum reaction conditions and the effect of additive substances were investigated. The composition of ion-association complexes was discussed also. Based on the ion-association reaction, a high sensitive, simple and rapid method was proposed for the determination of CTRX. The method was applied for the determination of CTRX in human serum and urine samples with satisfactory results.

Key words: ceftriaxone, acriflavine, fluorescence quenching

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