Synthesis of Oil-soluble Carbon Quantum Dots by Pyrolysis Method for the Detection of Oxytetracycline

doi:10.19894/j.issn.1000-0518.220340

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (4): 509-517.DOI: 10.19894/j.issn.1000-0518.220340

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Synthesis of Oil-soluble Carbon Quantum Dots by Pyrolysis Method for the Detection of Oxytetracycline

Yue-Xia ZHANG, Xiao-Peng FAN, Yu-Juan CAO, Xin-Tong YANG, Zhong-Ping LI, Zhen-Hua YANG(), Chuan DONG

Institute of Environmental Science，Shanxi University，Taiyuan 030006，China

Received:2022-10-19 Accepted:2023-02-20 Published:2023-04-01 Online:2023-04-17
Contact: Zhen-Hua YANG
About author:yzh429@sxu.edu.cn
Supported by:
Shanxi Scholarship Council of China(2020-002)

Abstract

Abstract:

Novel oil-soluble fluorescent carbon quantum dots （o-CDs） are prepared by pyrolysis with citric acid， glutathione and oleamine as raw materials. The o-CDs have good photochemical properties and optical stability. The maximum excitation wavelength and emission wavelengths are located at 375 and 440 nm， respectively， and the fluorescence quantum yield is 0.48. A fluorescence sensor with high sensitivity and selectivity for detection of oxytetracycline is established based on the fluorescence quenching effect. The o-CDs based fluorescence sensor exhibits a good linear relationship between the relative fluorescence intensity and the concentration of oxytetracycline （0.77~16.12 μg/mL） with the detection limit of 0.14 μg/mL. Furthermore， the sensor has the potential to determine oxytetracycline in milk samples. The spiked recoveries are in the range of 97.13%~104.18%， and the RSD value is less than 5%. The accuracy of the method is verified. The o-CDs is capable of monitoring oxytetracycline in food industry.

Key words: Pyrolysis method, Oil-soluble carbon dots, Oxytetracycline, Fluorescence quenching

CLC Number:

O655

Yue-Xia ZHANG, Xiao-Peng FAN, Yu-Juan CAO, Xin-Tong YANG, Zhong-Ping LI, Zhen-Hua YANG, Chuan DONG. Synthesis of Oil-soluble Carbon Quantum Dots by Pyrolysis Method for the Detection of Oxytetracycline[J]. Chinese Journal of Applied Chemistry, 2023, 40(4): 509-517.

Figures/Tables 10

Fig.1 Schematic representation of synthesis process of o-CDs and dispersibility of o-CDs in several solvents

Fig.2 Optimization of synthesis conditions of o-CDsNote： A. GSH amount； B. oleylamine dosage； C. reaction temperature；D. reaction time of GSH and CA； E. reaction time of oleylamine on the fluorescence intensity of o-CDs

Fig.3 （A） TEM image of o-CDs；（B） Size distribution of o-CDs

Fig.4 FT-IR spectra of N，S-CDs and o-CDs

Fig.5 （A） XPS full spectrum of o-CDs；（B） C1s XPS；（C） N1s XPS；（D） O1s XPS

Fig.6 （A） UV-Vis， excitation wavelengths and emission wavelength spectra of o-CDs；（B） Fluorescence emissionspectra of o-CDs with different excitation wavelengths

Fig.7 （A） The effect of different antibiotics on fluorescence intensity of o-CDs；（B） The influence of pH on fluorescence intensity of o-CDs sensors；（C） The effect of reaction time of OTC on fluorescence intensity of o-CDs

Fig.8 （A） Fluorescence spectra of o-CDs sensor for the detection of OTC；（B） The plot of theratios of （F0-F）/F0 versus the concentration of OTC；（C） Fitting diagram of fluorescence lifetime of o-CDs sensor

Table 1 Determination of OTC in milk by standard addition recovery method

Samples	Added/（μg·mL^-1）	Found/（μg·mL^-1）	Recovery/%	RSD/%
1	3.83	3.99	104.18	3.74
2	7.67	7.45	97.13	2.51
3	11.50	11.22	97.57	2.83

Table 2 Comparison of different methods for the detection of OTC

Method	Linear range	LOD	Ref.
HPLC	0.32~1000 μg/mL	6.07 μg/mL	［26］
SERS	0.2~22 μg/mL	0.2 μg/mL	［27］
SPE and AuNPs coated capillary electrophoresis	10~200 μg/mL	0.22 μg/mL	［28］
Electrochemical sensor， Biosensor	0.46~46.04 μg/mL	0.15 μg/mL	［4］
Up conversion luminescent nanomaterials	0.05~100 ng/mL	0.04 ng/mL	［29］
Up conversion luminescent nanomaterials	1~10 μg/mL	0.16 μg/mL	［30］
Fluorescence sensor	0.77~16.12 μg/mL	0.14 μg/mL	This work

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Synthesis of Oil-soluble Carbon Quantum Dots by Pyrolysis Method for the Detection of Oxytetracycline

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