Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (4): 518-526.DOI: 10.19894/j.issn.1000-0518.220397
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Rui ZHOU, Zi-Pin ZHANG(
)
Received:2022-12-09
Accepted:2023-03-07
Published:2023-04-01
Online:2023-04-17
Contact:
Zi-Pin ZHANG
About author:zpzhang@ahtcm.edu.cnSupported by:CLC Number:
Rui ZHOU, Zi-Pin ZHANG. A Novel Electrochemical Method for Berberine Detection in Biological Fluids[J]. Chinese Journal of Applied Chemistry, 2023, 40(4): 518-526.
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URL: http://yyhx.ciac.jl.cn/EN/10.19894/j.issn.1000-0518.220397
Fig.1 Chemical structures of berberine and BTB， and schematic illustration of the strategy for electrochemical detection of berberine based on poly-BTB modified electrode
Fig.2 Typical cyclic voltammograms （CVs） obtained at the SWNTs/GC electrode in BR buffer solution of pH=5.0 in the absence （a） and presence of 500 μmol/L berberine （b）. Scan rate is 50 mV/s
Fig.3 Successive 20-cycle CV at the BTB/SWNTs/GC electrode （A） and successive 50-cycle CV at poly-BTB/SWNTs/GC electrode （B） in BR buffer solution of pH=5.0. Scan rate is 50 mV/s. The red curve in Fig.3A represents the first cycle of the CV， and the arrows indicate the directions of the cycle numbers from 1 to 20 in Fig.3A. The insert shows the magnification of the 15th to 20 th cycles of the CVs in Fig.3A
Fig.4 （A） Typical CVs obtained at the poly-BTB/SWNTs/GC electrode （a， b） and SWNTs/GC electrode （c） in BR buffer solution of pH=5.0 before （a） and after （b， c） 20 min incubation with 500 μmol/L berberine. Scan rate is 50 mV/s；
Fig.5 Typical CVs obtained at the poly-BTB/SWNTs/GC electrode in BR buffer solution of pH=5.0 after incubation with 500 μmol/L berberine for 0， 1， 3， 5， 10， 15， 20， 25， 30 and 35 mins. Scan rate， 50 mV/s. The arrow indicates the direction of increasing of the incubation span. Inset the nonlinear plot of the ratio of current decrease versus incubation time
Fig.6 Typical DPVs obtained at poly-BTB/SWNTs-modified GC electrode in BR buffer solution of pH=5.0 upon addition of different concentrations of berberine. The arrow indicates the direction of increasing concentration of berberine. Inset： linear plot of the ratio of current decrease versus concentration of berberine in the ranges of 0.05~1 μmol/L and 1~100 μmol/L
Fig.7 （A） Typical DPVs obtained at the poly-BTB/SWNTs-modified GC electrodes in BR buffer solution of pH=5.0 in the absence （a） and the presence of 10 μL blank plasma （b）， and 10 μL model plasma of rats after dosing of berberine for 1 h （c） and 2 h （d）. （B） Typical DPVs obtained at the poly-BTB/SWNTs-modified GC electrodes in BR buffer solution of pH=5.0 in the absence （a） and the presence of 10 μL blank liver homogenate （b） and 10 μL model plasma of rats after dosing of berberine for 3 h （c）
| Sample | Spiked/（μmol·L-1） | Found/（μmol·L-1） | Recovery/% | RSD/% |
|---|---|---|---|---|
| Plasma | 0.20 | 0.20 | 100.0 | 2.5 |
| 20 | 20.64 | 103.2 | 1.9 | |
| Liver homogenate | 50 0.20 20 50 | 51.08 0.20 19.37 50.49 | 102.2 101.5 96.9 100.9 | 2.0 4.6 3.2 1.6 |
Table 1 Spike-and-recovery in plasma and liver homogenate（n=3）
| Sample | Spiked/（μmol·L-1） | Found/（μmol·L-1） | Recovery/% | RSD/% |
|---|---|---|---|---|
| Plasma | 0.20 | 0.20 | 100.0 | 2.5 |
| 20 | 20.64 | 103.2 | 1.9 | |
| Liver homogenate | 50 0.20 20 50 | 51.08 0.20 19.37 50.49 | 102.2 101.5 96.9 100.9 | 2.0 4.6 3.2 1.6 |
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