Chinese Journal of Applied Chemistry

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Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin

YANG Shaoming*, ZHA Wenling, SUN Qin, LI Hong, LI Ruiqin, SHANG Peiling   

  1. (Department of Chemistry and Chemical Engineering,School of Sciences,
    East China Jiaotong University,Nanchang 330013,China)
  • Received:2013-08-16 Revised:2013-10-10 Published:2014-06-10 Online:2014-06-10
  • Contact: wenling

Abstract: A label-free electrochemical aptasensor has been constructed for the electrochemical detection of thrombin. Two layers of nickel hexacyanoferrate redox probes sandwiched with multi-walled carbon nanotubes for the sensitivity enhancement were directly immobilized on the electrode surface by electrodeposition, and gold nanoparticles was used as the immobilization matrix for the aptamers. SEM was used to characterize the morphologies of multi-walled carbon nanotubes and nickel hexacyanoferrate. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of aptamer biosensors. Electrochemical behaviors of aptamer biosensors were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor shows a good linear range of 1.0 ng/L~1.0 mg/L(R=0.998) for the electrochemical detection of thrombin with a detection limit of 0.2 ng/L(S/N=3).

Key words: nickel hexacyanoferrate, aptamer, thrombin, biosensor

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