Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (1): 28-39.DOI: 10.11944/j.issn.1000-0518.2018.01.170363

• Review • Previous Articles     Next Articles

Aptamer-based Fluorescence Probe for Bioanalysis and Bioimaging

HUANG Zike,LIU Chao,FU Qiangqiang,LI Jin,ZOU Jianmei,XIE Sitao,QIU Liping()   

  1. State Key Laboratory of Chemo/Bio-Sensing and Chemometrics,Hunan University,Changsha 410082,China
  • Received:2017-10-13 Accepted:2017-11-24 Published:2018-01-03 Online:2018-01-03
  • Contact: QIU Liping
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21505039)


Aptamers are oligonucleotides selected through an in vitro method known as systematic evolution of ligands by exponential enrichment(SELEX), which can recognize target with high specificity and high affinity. Aptamers have attracted wide attention because of their intrinsic advantages including simple synthesis, low molecular mass, high chemical stability, easy biochemical modification and so on. Aptamers can fold into specific two-dimensional or three-dimensional configurations and specifically bind with their targets. With appropriate signal transduction mechanism, aptamers can provide ideal molecular recognition and detection probes for biological research. Fluorescence is a type of detection technology that has several superiorities of high sensitivity, high resolution and easy multiplex detection. With the combination of the molecular recognition capability of aptamers and the outstanding detection performance of fluorescence technology, the aptamer-based fluorescence probes have been widely used in the study of life science. This review highlights the major progress of aptamer-based fluorescence probes in both bioanalysis and bioimaging area, and discusses the current limitations of aptamer probe for applications.

Key words: aptamer, fluorescence probe, bioanalysis, bioimaging