Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (2): 212-222.DOI: 10.11944/j.issn.1000-0518.2019.02.180161

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Synthesis of 1,2,4-Triazine-PhenanthrolineCo(Ⅲ) Complexes and Their FluorescenceRecognition on Calf Thymus DNA

ZHANG Chenglu(),WANG Jing,LI Jingyi,YU Xiangkun,YANG Jingyi,CAI Jinhua,LI Yizhen,WANG Huayu,GONG Rongqing   

  1. College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian,Liaoning 116029,China
  • Received:2018-05-08 Published:2019-02-01 Online:2019-01-31
  • Contact: ZHANG Chenglu
  • Supported by:
    Supported by Technology Research Program of Liaoning Provincial Department of Education(No.2009A426)


Four 1,2,4-triazine-phenanthroling derivatives(ARTP1ARTP4) were designed and synthesized successfully. ARTP1ARTP4 were selected to coordinate with Co3+ to afford four novel complexes(Co-ARTP1Co-ARTP4). The structures and properties of ARTP1ARTP4 and Co-ARTP1Co-ARTP4 were studied by means of Fourier transform infrared spectrometer(FT-IR), nuclear magnetic resonance(NMR), high resolution mass spectrometry(HRMS) and ultraviolet-visible spectroscopy(UV-Vis). As a result, the excitation peaks of the complexes are weakened and show red shift. The complexes interact with calf thymus DNA(CT-DNA) through the insert mode and the binding constants are Kb=4.78×105, 6.52×105, 5.97×105 and 6.01×105 L/mol, respectively, which indicates that the complexes are expected to be the DNA fluorescence probes. It provides the important theory references for further study on the interaction between small organic molecules with DNA.

Key words: phenanthroline, 1,2,4-triazine, calf thymus DNA, fluorescence probe, Co-complexes