Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (7): 830-838.DOI: 10.11944/j.issn.1000-0518.2020.07.190318

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Simple, Fast and Accurate Detection of Ciprofloxacin Based on Fluorescence Enhancement of Nitrogen-Doped Carbon Dots

XU Lipinga, LIU Qingshia, DONG Zhichena, GUO Xingjiaa, DONG Weib,*   

  1. aCollege of Chemistry,Liaoning University,Shenyang 110036,China;
    bDepartment of Chemistry,Shenyang Medical College,Shenyang 110034,China
  • Received:2019-11-26 Published:2020-07-01 Online:2020-07-07
  • Contact: DONG Wei, professor; Tel:024-62207809;; Research interests:synthesis and application of fluorescent carbon dots
  • Supported by:
    Supported by the Natural Science Foundation of Liaoning Province(No.20180550105)

Abstract: Nitrogen doped fluorescence carbon dots (N-CDs) were successfully synthesized by a facile one step solid phase pyrolysis treatment using malic acid as the carbon source and ammonium phosphate as the nitrogen source. The fluorescence quantum yield of the obtained N-CDs reaches 20.7% and the N-CDs have approximately spherical morphology with an average diameter of 3.3 nm. The as prepared N-CDs were used as the fluorescent probe to detect ciprofloxacin (CIP) based on the enhancement of N-CDs fluorescence upon adding CIP. The optimal experimental mass conditions are 7.5 μg/mL of N-CDs, pH=5.91, and 5 min of incubation time for the detection of CIP. The enhancement of N-CDs fluorescence exhibits a good linear relationship with the concentration of CIP ranged from 0.39 μmol/L to 40.0 μmol/L under the optimal conditions. The linear regression equation is ΔF=1.61×107[CIP]-3.28 with a correlation coefficient R2=0.994. The detection limit and relative standard deviation (n=5) are estimated to be 0.12 mol/L and 4.2%, respectively. The interference experiments indicate that potential coexistence substances (except copper ions) have ignorable effects on the detection of CIP, and the interference of copper ions could be masked with 4% ammonium oxalate solution. Finally, the proposed sensor was successfully applied to analyze real samples with satisfactory results.

Key words: nitrogen doped carbon dots, ciprofloxacin, fluorescence probe, fluorescence enhancement