Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (11): 1324-1332.DOI: 10.11944/j.issn.1000-0518.2020.11.200125

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Determination of Aflatoxin B1 in Food by LC-MS/MS Method Based on Magnetic Bead-Aptamer

GU Xinyua, LANG Leb*, WANG Jianweic, ZHAO Lihuia*   

  1. aCollege of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China;
    bJilin Institute for Food Control,Changchun 130103,China;
    cNational Analytical Research Center of Electrochemistry and Spectroscopy,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
  • Received:2020-04-29 Revised:2020-06-09 Accepted:2020-07-08 Online:2020-11-01 Published:2020-11-04
  • Contact: ZHAO Lihui, professor; Tel/Fax:0431-85583891;; Research interests:synthesis of nanomaterials, diagnosis and treatment of tumors
    Co-corresponding author:LANG Le, associate professor; Tel/Fax:0431-88675932;; Research interests:food quality and safety

Abstract: In this study, a magnetic bead-aptamer that specifically recognizes aflatoxin B1 (AFB1) was constructed and coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) to establish a quantitative detection method for AFB1 in food. Carboxy magnetic beads were activated by carbodiimide hydrochloride (EDC) and incubated with 5'terminal amino modified aptamers. The aptamers were covalently linked to the surface of the carboxy magnetic beads by amidation reaction and used as capture probes to separate AFB1 in the extracted sample. The qualitative and quantitative analysis of AFB1 was carried out by LC-MS/MS. The results show that the method has a good linear relationship at the concentration of 0.25~25 ng/mL with the correlation coefficient R2=0.999, the quantitative detection limit is 0.25 ng/mL, the recovery is 80.3%~92.5%, and the relative standard deviation (RSD) is less than 8%. The method is simple, fast and convenient, and can detect trace AFB1. The magnetic bead aptamer can be reused, which provides another technical support for the quantitative detection of AFB1.

Key words: magnetic beads, aptamers, LC-MS/MS, aflatoxin B1

CLC Number: