Microcantilever Biosensor for Detection of Adenosine Triphosphate

doi:10.11944/j.issn.1000-0518.2015.03.140437

Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (3): 362-366.DOI: 10.11944/j.issn.1000-0518.2015.03.140437

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Microcantilever Biosensor for Detection of Adenosine Triphosphate

LI Huiyan^{a, b}, BAI Xiaojing^b, WANG Fenghong^a, LI Jing^{a, *}, TANG Jilin^b

^aDepartment of Health Inspection,School of Public Health,Jilin University,Changchun 130021,China
^bState Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China

Received:2014-12-22 Accepted:2015-01-16 Published:2015-03-02 Online:2015-03-02
Contact: Jing LI
Supported by:

Abstract

Abstract:

An aptamer-based microcantilever biosensor for label-free detection of adenosine triphosphate(ATP) was developed. The sensor cantilevers in the microcantilever array were functionalized with ATP aptamer on the gold side to recognize ATP, while the reference cantilevers were modified by 6-mercapto-1-hexanol(MCH) to prevent non-specific absorption. The interaction between ATP and aptamer on the sensor cantilerers gold surface induced surface stress changes, resulting in a differential deflection between the sensor and reference cantilevers. The differential deflection has a good linear relationship with the ATP concentration ranging from 0.5~5 mmol/L with a correlation coefficient of 0.998. The detection limit is about 0.06 mmol/L. The aptamer-based microcantilever sensor shows rapid response, simple procedures and high specificity for ATP.

Key words: adenosine triphosphate, aptamer, microcantilever biosensor, surface stress

CLC Number:

LI Huiyan, BAI Xiaojing, WANG Fenghong, LI Jing, TANG Jilin. Microcantilever Biosensor for Detection of Adenosine Triphosphate[J]. Chinese Journal of Applied Chemistry, 2015, 32(3): 362-366.

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Microcantilever Biosensor for Detection of Adenosine Triphosphate

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