Preparation of Graphene Nano-Drug Carrier System and Its Killing Effect on Tumor Cells

doi:10.19894/j.issn.1000-0518.200302

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (6): 693-703.DOI: 10.19894/j.issn.1000-0518.200302

• Full Papers • Previous Articles Next Articles

Preparation of Graphene Nano-Drug Carrier System and Its Killing Effect on Tumor Cells

ZHANG Ya-Jing¹, WANG Xiao-Hui³, XU Ya-Juan⁴, LIU Wen-Shu^2*, ZHAO Li-Hui^1*

¹College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
²Department of Stomatology, the First Clinical Hospital of Jilin University, Changchun 130012, China
³Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
⁴Oral and Maxillofacial Surgery, Jilin Cancer Hospital, Changchun 130012, China

Received:2020-10-06 Revised:2020-12-16 Published:2021-06-01 Online:2021-08-01
Supported by:
Science and Technology Department of Jilin Province (No.JJKH20170604KJ)

Abstract

Abstract: In this study, a nano-graphene oxide (NGO) was modified by polyethylene glycol (PEG) and branched polyethyleneimine (bPEI) as a drug loading platform of macromolecular drugs, which increases the water solubility of NGO and the adsorption of proteins. Then, tumor patient serum was prepared by loading anti-cancer drug cis-diamminedichloroplatinum (CDDP) and low-temperature ethanol, respectively, to form the graphene nanoparticle drug delivery system. The ultraviolet-visible and Fourier transform infraredspectroscopy characterization results demonstrate that the NGO-PEG-bPEI-CDDP drug loading system is successfully prepared, and the drug loading rate of NGO-PEG-bPEI to CDDP is 34.6%. Polyacrylamide gel electrophoresis (SDS-PAGE) demonstrates strong adsorption of specific antibody proteins in the NGO-PEG-bPEI-CDDP-Antibody macromolecular nano-loading system, and the nanocomposite can be specifically enriched in tumors. Cell sites are extremely sensitive to the recognition of CNE-1 cell lines. Cytotoxicity test (MTT) results demonstrate that the drug delivery system of NGO-PEG-bPEI-CDDP-Antibody macromolecular nano graphene can kill KB and CNE-1 cells and avoid additional damage to normal cells at the same concentration and time. The NGO-PEG-bPEI-CDDP-Antibody nano drug-loading system can not only enrich the drug in the lesion area through specific recognition, reduce normal cell damage, but also effectively kill cancer cells and reduce the dose of chemotherapy drugs, which is a very promising macromolecular nano-drug loading system.

Key words: Nano-graphene oxide, Macromolecular drug loading system, Tumor-specific antibody, Tumor enrichment

CLC Number:

O611

ZHANG Ya-Jing, WANG Xiao-Hui, XU Ya-Juan, LIU Wen-Shu, ZHAO Li-Hui. Preparation of Graphene Nano-Drug Carrier System and Its Killing Effect on Tumor Cells[J]. Chinese Journal of Applied Chemistry, 2021, 38(6): 693-703.

References

[1] LIN Z R, XU Y Y, ZHANG T. A theoretical exploration into durability of carbon nanotubes cement-based materials[J]. Low Temp Architect Technol, 2011, 2(3): 996-1000.
[2] BANERJEE A N. Graphene and its derivatives as biomedical materials: future prospects and challenges[J]. Interface Focus, 2018, 8(3): 1-22.
[3] RADUNOVIC M, DE C M, DE M P, et al. Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence[J] . J Biomed Mater Res Part A, 2017, 105(8): 2312-2320.
[4] LI Y, LU Q, LIU H, et al. Antibody-modified reduced graphene oxide films with extreme sensitivity to circulating tumor cells[J]. Adv Mater, 2015, 27(43): 6848-6854.
[5] ZHANG C, LIU Z, ZHENG Y, et al. Glycyrrhetinic acid functionalized graphene oxide for mitochondria targeting and cancer treatment in vivo[J]. Small, 2018, 14(4): 1703306.
[6] 梁爽, 韩雷强, 张娜. 结构修饰的基于聚乙烯亚胺的载体在基因递送方面的研究进展[J]. 药物生物技术, 2017, 10(4): 55-61.
LIANG S, HAN L Q, ZHANG N. Research progress of structurally modified polyethyleneimine-based carriers in gene delivery[J]. Pharm Biotechnol, 2017, 10(4): 55-61.
[7] 武叙, 李晓宇, 姚翀, 等. BPEI/miRNA, PAMAM/miRNA和PEI/miRNA治疗前列腺癌作用研究[J]. 中国药学杂志, 2015, 50(9): 768-774.
WU X, LI X Y, YAO C, et al. Effect of BPEI/miRNA, PAMAM/miRNA and PEI/miRNA delivery system on the treatment of prostate cancer[J]. J Chinese Pharm, 2015, 50(9): 768-774.
[8] OU M, WANG XL, XU R, et al. Novel biodegradable poly(disulfide amine)s for gene delivery with high efficiency and low cytotoxicity[J]. Bioconjugate Chem, 2008, 19(3): 626-633.
[9] 杨传孝, 余梦雯, 宋朵朵, 等. 甲醛功能化聚乙烯亚胺-罗丹明B酰肼比率及可视化荧光测定Cr(Ⅵ)[J]. 高等学校化学学报, 2016, 37(5): 852-859.
YANG C X, YU M W, SONG D D, et al. Modification of formaldehyde-functionalized polyethyleneimine-rhodamine B hydrazide and its visual fluorescence spectrometry for Cr(VI)[J]. Chem J Chinese Univ, 2016, 37(5): 852-859.
[10] ZHANG L, LU Z, ZHAO Q, et al. Enhanced chemotherapy efficacy by sequential delivery of siRNA and anticancer drugs using PEI-grafted graphene oxide[J]. Small, 2011, 7(4): 460-464.
[11] ZHOU T, ZHOU X, XING D. Controlled release of doxorubicin from graphene oxide based charge-reversal nanocarrier[J]. Biomaterials, 2014, 35 (13): 4185-4194.
[12] WENG Y, JIANG B, YANG K, et al. Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization[J]. Nanoscale, 2015, 7(34): 14284-14292.

[1]	Ning YUAN, Jie MA, Jin-Ling ZHANG, Jian-Sheng ZHANG. Preparation of PCN-6（M） Bimetallic Organic Framework Materials by Steam-assisted Method and Their CO₂ and CH₄ Adsorption Performance [J]. Chinese Journal of Applied Chemistry, 2023, 40(6): 896-903.
[2]	Lu-Fei WANG, Meng-Meng ZHEN, Bo-Xiong SHEN. Research Progress of Controlling Lithium-Sulfur Batteries by Electrocatalysts under Lean Electrolyte Conditions [J]. Chinese Journal of Applied Chemistry, 2023, 40(2): 188-209.
[3]	Zhi-Peng DU, Yang ZHOU, San-Gen ZHAO. Synthesis， Crystal Structure and Birefringence Properties of Silver Cluster Compound Ag₃B₆O₁₀I [J]. Chinese Journal of Applied Chemistry, 2023, 40(2): 229-235.
[4]	Hui LU, Jiang LI, Li-Hua WANG, Ying ZHU, Jing CHEN. Researsh Progress of Photocatalytic Applications of Atomically Precise Coinage Metal Nanoclusters [J]. Chinese Journal of Applied Chemistry, 2022, 39(11): 1652-1664.
[5]	Xue-Xian YANG, Jian ZHANG, Zhi-Gang GU. Surface‑Coordinated Metal‑Organic Framework Thin Film HKUST‑1 for Optoelectronic Applications [J]. Chinese Journal of Applied Chemistry, 2022, 39(7): 1013-1025.
[6]	Qian ZHU, Xiang-Yan HOU, Ke-Ke HUANG. Antitumor Nanomaterials [J]. Chinese Journal of Applied Chemistry, 2022, 39(6): 1011-1012.
[7]	Mei-Lun ZHANG, Hong-Ran LING, Zai-Chun SUN, Bing-Chu MEI, Wei-Wei LI. Research Progress on the Preparation Methods of BaMgF₄ Powders [J]. Chinese Journal of Applied Chemistry, 2022, 39(6): 900-911.
[8]	Wei-Jin CAO, Lu BAI, Lan-Lan WU, Jing-De LI, Shu-Yan SONG. Multi⁃Shell Hollow Nickel⁃Cobalt Bimetallic Phosphide Nanospheres for Highly Efficient Oxygen Evolution Reaction [J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 666-672.
[9]	WANG-Xin, ZHANG-Dong, DU-Fei. Recent Progress of Single⁃Atom Catalytic Materials for Lithium⁃Sulfur Batteries [J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 513-527.
[10]	Qi ZHANG, Qian ZHANG, Xiao-Meng SHI, Ya-Qi KONG, Ke-Xin GAO, Ya-Ping DU. Research Progress of Rare Earth Bromides Based Solid Electrolytes for All⁃Solid⁃State Batteries [J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 585-598.
[11]	Yi-Ying ZHANG, Cui-Yan LI, Jie ZHAO, Xiao-Ming YU, Qian-Rong FANG. Porphyrin⁃Sulfide⁃Based Covalent Organic Frameworks as Oxygen Reduction Reaction Electrocatalysts [J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 647-656.
[12]	Xiao-Feng WU, De-Shun CHEN, Wei MA, Ke-Ke HUANG. WO₃/Fe₂TiO₅ Composite Photoanode Deposited via Electrospray for Enhanced Photoelectrochemical Water Splitting [J]. Chinese Journal of Applied Chemistry, 2022, 39(4): 694-696.
[13]	Chun-Mei ZHAO, Xiu-Miao ZHOU, Xi-Xi JIN, Yu-Hang WANG, Yi-Jing DANG. Preparation of Saddle-Shaped Cyclooctatetrathiophene-Based Hybrid Nanomaterials and Fluorescence Properties [J]. Chinese Journal of Applied Chemistry, 2022, 39(02): 283-288.
[14]	ZHU Meng-Meng, BAI Jue-Yao, CHEN Ren-Jie, LI Hui-Li. Synthesis and Properties of B-Site Doped All-Inorganic Perovskite Quantum Dots [J]. Chinese Journal of Applied Chemistry, 2021, 38(12): 1541-1555.
[15]	WANG Sen, PANG Ran, LI Da, LI Cheng-Yu, ZHANG Hong-Jie. Synthesis and Luminescence Properties of a Single-Phase White-Emitting and Tunable Color Phosphor Na₃Sc₂(PO₄)₃∶Tm³⁺,Dy³⁺ [J]. Chinese Journal of Applied Chemistry, 2021, 38(11): 0-0.

Preparation of Graphene Nano-Drug Carrier System and Its Killing Effect on Tumor Cells

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments