Yellow Fluorescent Carbon Dots for Sensitive Detection of Chlortetracycline Hydrochloride

doi:10.19894/j.issn.1000-0518.220386

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (7): 1017-1023.DOI: 10.19894/j.issn.1000-0518.220386

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Yellow Fluorescent Carbon Dots for Sensitive Detection of Chlortetracycline Hydrochloride

Pan YU, Guang-Hui WANG, Jian-Hua GUO, Qiao-Zhi GUO, Chuan DONG()

School of Chemistry and Chemical Engineering，Institute of Environmental Science，Shanxi University，Taiyuan 030006，China

Received:2022-11-29 Accepted:2023-05-05 Published:2023-07-01 Online:2023-07-19
Contact: Chuan DONG
About author:dc@sxu.edu.cn
Supported by:
the National Natural Science Foundation of China(22274090)

Abstract

Abstract:

Yellow fluorescent carbon dots （Y-CDs） are prepared by one-step hydrothermal method using o-phenylenediamine and L-tyrosine as raw materials， which can be employed as a fluorescence probe for the determination of chlortetracycline hydrochloride （CTC）. In the presence of chlortetracycline hydrochloride， the fluorescence emission of Y-CDs is dramatically quenched due to internal filtration effect or static quenching effect. Therefore， a highly sensitive and selective fluorescence method is developed for the detection of CTC. The fluorescent intensity of Y-CDs has a good linear relationship with CTC in the range of 2.5~145 μmol/L and the detection limit of 1.43 μmol/L. The fluorescence probe is further applied to the detection of CTC in the milk and tap water with the recoveries in the range of 97.49%~106.90%.

Key words: Yellow fluorescent carbon dots, Fluorescence probe, Chlortetracycline hydrochloride

CLC Number:

O655

Pan YU, Guang-Hui WANG, Jian-Hua GUO, Qiao-Zhi GUO, Chuan DONG. Yellow Fluorescent Carbon Dots for Sensitive Detection of Chlortetracycline Hydrochloride[J]. Chinese Journal of Applied Chemistry, 2023, 40(7): 1017-1023.

Figures/Tables 7

Fig.1 Principle illustration of the synthetic procedure of Y-CDs and the mechanism of detection of chlortetracycline hydrochloride

Fig.2 （A） TEM image of the Y-CDs， the upper right illustration shows the distribution of particle size；（B） FT-IR spectrum of Y-CDs；（C） XPS spectrum of Y-CDs；（D） C1s；（E） N1s；（F） O1s， high resolution XPS patterns of Y-CDs

Fig. 3 （A） UV-Vis absorption， fluorescence spectra of Y-CDs in aqueous solution；（B） Fluorescence emission spectra of the Y-CDs under different excitation wavelengths

Fig.4 （A） Fluorescence intensity of Y-CDs at different concentration of NaCl；（B） Fluorescence intensity of Y-CDs under continuous irradiation by xenon lamp；（C） Fluorescence intensity of 0.1 mg/mL Y-CDs under different pH；（D） The zeta potential of Y-CDs

Fig.5 （A） Fluorescence responses of the Y-CDs in the presence of small biomolecules；（B） Fluorescence responses of the Y-CDs toward different concentrations of CTC；（C） Linear relationship between F0/F and CTC concentration

Fig. 6 （A） The mechanisms of CTC detection by the Y-CDs. UV spectrum of CTC， and the maximum excitation and emission fluorescence spectra of Y-CDs；（B） UV-Vis absorption spectrum of CTC， Y-CDs， Y-CDs + CTC aqueous solution；（C） PL decay curves of Y-CDs aqueous solution and Y-CDs+CTC compound aqueous solution；（D） The linear relation between （F0-F）/F and CTC concentration

Table 1 Determination of CTC in milk and tap water

	Spiked/（μmol·L^-1）	Found/（μmol·L^-1）	Recovery/%（n=6）	RSD/%（n=6）
Milk	10	10.43	104.30	3.68
	55	54.65	99.34	4.01
	70	70.27	100.39	3.13
Tap water	10	9.74	97.49	1.36
	55	58.03	105.52	3.24
	70	74.83	106.90	1.96

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Yellow Fluorescent Carbon Dots for Sensitive Detection of Chlortetracycline Hydrochloride

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