Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (5): 595-602.DOI: 10.11944/j.issn.1000-0518.2019.05.180310

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A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction

WEI Guobing,KONG Derong,XU Huihui,YIN Zhaojiang,ZHANG Jing,CUI Hanfeng,HONG Nian,YANG Jie,XIONG Wei,LIU Wenming,GUO Qianwen,CHENG Lin(),FAN Hao()   

  1. Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China
  • Received:2018-09-20 Accepted:2019-01-23 Published:2019-05-01 Online:2019-05-06
  • Contact: CHENG Lin,FAN Hao
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.81660658, No.81860682, No.81560625, No.81872968), Jiangxi Youth Natural Science Foundation(No.20161BAB215212), Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission(No.2017A280), Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine(No.JZYC17S07, No.JZYC18S13), Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine(No.2017jzzdxk036), Science and Technology Project of Jiangxi Education Department(No.GJJ160833)


A new type of “Off-On” electrochemiluminescence(ECL) biosensor using Ru(bpy)32+/β-CD-AuNPs/Nafion complex and ferrocene-labeled DNA(Fc-DNA) was designed to detect mercury ions in Chinese medicinal materials Danshen. The ECL biosensor includes an ECL substrate and an ECL signal switch. Ru(bpy)32+/β-CD-AuNPs/Nafion complex modified glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)32+, and hairpin-like Fc-DNA probe acted as the ECL signal switch, which was designed by molecular recognition strategy and attached to β-CD-AuNPs through host-guest interaction between β-CD and ferrocene . Compared with previous methods of mercury ion detection, this method has the advantages of convenient operation, high sensitivity, good repeatability and low detection limit. The ECL biosensor offered good linear responses for Hg2+ in the range of 0.04~800 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).

Key words: electrochemiluminescence, biosensor, mercury(Ⅱ), molecular recognition technology, Switch