Dual Mode Detection of Chlorogenic Acid Based on Silicon Quantum Dot via Fluorescence Spectroscopy and Colorimetric Method

doi:10.19894/j.issn.1000-0518.240394

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (4): 511-521.DOI: 10.19894/j.issn.1000-0518.240394

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Dual Mode Detection of Chlorogenic Acid Based on Silicon Quantum Dot via Fluorescence Spectroscopy and Colorimetric Method

Song-Bai WANG¹(), Tian-Yu LI¹, Meng-Shu WEI¹, Xin-Ming GUO¹, Jun-Fen LI¹, Ying ZHOU¹, Xiu-Ying PENG², Chuan DONG¹()

^1.School of Chemistry and Chemical Engineering，Institute of Environmental Science，Shanxi University，Taiyuan 030006，China
^2.Department of Environment and Safety Engineering，Taiyuan Institute of Technology，Taiyuan 030008，China

Received:2024-11-30 Accepted:2025-03-17 Published:2025-04-01 Online:2025-05-14
Contact: Song-Bai WANG,Chuan DONG
Supported by:
Shanxi Scholarship Council of China(2021-011);the Basic Research Project of Shanxi Provincial(202103021223351)

Abstract

Abstract:

A silicon quantum dots （SiQDs） was prepared by refluxing 3-aminopropyltriethoxysilane （APTES）， sodium ascorbate and sodium citrate with heating at 90 ℃. Based on the SiQDs， an efficient， sensitive and convenient method for the detection of chlorogenic acid （CGA） was constructed based on the dual-mode fluorescence and UV absorption via electrostatic adsorption between SiQDs and CGA. The SiQDs possessed good water solubility as well as excellent selectivity for CGA. The fluorescence quantum yield （QD） of SiQDs was as high as 0.58. Under the optimal working conditions， the linear range of fluorescence detection of SiQDs was 0.054~265 μmol/L， and the limit of detection was 0.018 μmol/L. The absorbance detection of SiQDs was 0.516~45 μmol/L， and the limit of detection was 0.172 μmol/L. In addition， the analytical detection of CGA in Lonicera japonica Thunb. was realized by using the spiked recovery method， which showed good recoveries （97.76%~105.5%）.

Key words: Silicon quantum dots, 3-Aminopropyltriethoxysilane, Chlorogenic acid, Fluorescent probe

CLC Number:

O655

Song-Bai WANG, Tian-Yu LI, Meng-Shu WEI, Xin-Ming GUO, Jun-Fen LI, Ying ZHOU, Xiu-Ying PENG, Chuan DONG. Dual Mode Detection of Chlorogenic Acid Based on Silicon Quantum Dot via Fluorescence Spectroscopy and Colorimetric Method[J]. Chinese Journal of Applied Chemistry, 2025, 42(4): 511-521.

Figures/Tables 10

Fig.1 Construction of SiQDs fluorescence and colorimetric sensors for detection of chlorogenic acid

Fig.2 （A） TEM，（B） histogram of particle size distribution，（C） XRD pattern and （D） FT-IR spectrum of SiQDs

Fig.3 （A） XPS mapping of SiQDs；（B-E） C1s， N1s， O1s and Si2p mapping

Fig.4 （A） Fluorescence emission spectra of SiQDs at different excitation wavelengths；（B） UV-Vis absorption spectrum of SiQDs；（C） Effect of room temperature placement time on the fluorescence intensity of SiQDs；（D） Effect of pH on the fluorescence intensity of SiQDs；（E） Effect of excitation time on the fluorescence intensity of SiQDs；（F） Effect of ionic strength on the fluorescence intensity of SiQDs

Fig.5 （A） Effect of reactant ratio on experimental results；（B） Effect of reaction time on experimental results；（C） Effect of reaction temperature on experimental results；（D） Line graph of Fig.5A；（E） Line graph of Fig.5B；（F） Line graph of Fig.5C

Fig.6 （A） Fluorescence intensity of SiQDs in the presence of ionic interferences Br-， Zn2+， Cu2+， Fe2+， NO2-， Pb2+，SO42-， Al3+， K+， Cl-（1500 μmol/L） and the addition of 150 μmol/L CGA；（B） Fluorescence intensity of SiQDs in the presence of organic small molecule interferences Gln， L-Cys， L-Tyr， SA， AA， GSH， Met， CA， DA， Glu， Try， Glucose， Sucrose， Leu， THB， QA and CAA （1500 μmol/L） in the presence of 150 μmol/L CGA；（C） Fluorescence spectrograms of SiQDs with the concentration of CGA ranging from 0 to 435 μmol/L；（D） Linear relationship between ln （FI） and CGA concentration

Fig.7 （A） Absorbance of SiQDs in the presence of ionic interferences Cu2+， NO2-， Zn2+， Cl-， Br-， Fe2+， Al3+， NO3-， Pb2+， K+ and SO42-（500 μmol/L） and with the addition of 50 μmol/L CGA；（B） Absorbance of SiQDs in the presence of organic small molecule interferences L-Tyr， Glucose， Sucrose， DA， GSH， Gln， SA， Met， CA， Cys， Glu， Try， Leu， THB， QA， CAA （500 μmol/L） and the addition of 50 μmol/L CGA；（C） UV profiles of SiQDs in the range of 0~1000 μmol/L for the concentration of CGA；（D） Linear plots of the absorbance and the concentration of CGA

Table 1 Comparison of this method with other CGA detection methods

Detection method	Material	Linear range/（μmol·L^-1）	LOD of CGA/（μmol·L^-1）	Ref.
Electrochemistry	TAPB-DMTP-COFS/AuNPs/GCE	0.010~40	0.009 5	［26］
	BDD	5.64~147.0	1.260	［27］
	MIS/Au/GCE	0.50~14	0.148	［28］
	PASA/GCE	0.40~12	0.040	［29］
Fluorescence	CDs	0.15~60	0.045	［25］
	CDs	0.10~220	0.030	［24］
	CDs	1.53~80	0.460	［30］
	SiQDs	0.054~265	0.018	This work
Colorimetry	Probe	11.3~113	0.475	［31］
	SiQDs	0.516~45	0.172	This work

Fig.6 （A） Fluorescence decay curves of SiQDs （τ0） and SiQDs+CGA （τ）；（B） Zeta potentials of SiQDs （1 mg/mL）， APTES （0.06 mol/L）， CGA （0.01 mol/L）， and SiQDs+CGA and APTES+CGA；（C） TEM images of SiQDs incorporated into CGA （inset： histogram of frequency distribution of particle sizes）；（D） UV-Vis absorption spectra of SiQDs （1 mg/mL）， CGA （0.01 mol/L）， and SiQDs+CGA

Table 2 Determination of CGA in Lonicera japonica Thunb.

Samples	Found/（μmol·L^-1）	Add/（μmol·L^-1）	Total found/（μmol·L^-1）	Recovery/%	Average recovery/%	RSD/%
Lonicera japonica Thunb.	0.82	89.29	89.23	99.02	98.55	0.56
	0.85	89.29	88.12	97.76
	0.83	89.29	89.10	98.87
	8.97	44.44	54.70	102.4	102.8	1.96
	9.01	44.44	56.28	105.5
	8.91	44.44	53.68	100.6
	27.09	17.67	46.48	103.8	102.9	2.18
	26.12	17.67	43.69	99.77
	25.66	17.67	45.51	105.0

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Dual Mode Detection of Chlorogenic Acid Based on Silicon Quantum Dot via Fluorescence Spectroscopy and Colorimetric Method

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