Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (9): 1053-1060.DOI: 10.11944/j.issn.1000-0518.2019.09.190023

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A Coumarin-Based Fluorescent Probe for Rapid Detection of Endogenous Formaldehyde

GAO Man,HE Xin,CUI Jingnan(),LIU Tao,TIAN Zhenhao,HE Shengui   

  1. State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian,Liaoning 116024,China
  • Received:2019-01-22 Accepted:2019-04-23 Online:2019-09-05 Published:2019-09-05
  • Contact: Jingnan CUI
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21572029)


A coumarin-based formaldehyde(FA) fluorescent probe Coun was designed and synthesized. The mechanism of this fluorescent probes for FA is based on blocking electron transfer between hydrazine and the dye upon FA nucleophilic addition. The results show that the optimal reaction time of Coun and FA is 4 min, which greatly improved the detection efficiency with detection limit of 5×10-6 μmol/L. In addition, the probe has good cell membrane permeability, and can be used in the biological field to detect endogenous formaldehyde.

Key words: fluorescent probe, formaldehyde, fast response, cell imaging