Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (11): 1726-1734.DOI: 10.19894/j.issn.1000-0518.220041

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Neodymium and Nitrogen Co‑doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging

Jin-Ping SONG1, Qi MA1(), Xiao-Min LIANG2, Jian-Peng SHANG1, Chuan DONG()   

  1. 1.College of Chemistry and Chemical Engineering,Institute of Applied Chemistry,Shanxi Datong University,Datong 037009,China
    2.School of Chemistry and Material Science,Shanxi Normal University,Linfen 041004,China
    3.Institute for Environmental Science,Shanxi University,Taiyuan 030006,China
  • Received:2022-02-21 Accepted:2022-04-20 Published:2022-11-01 Online:2022-11-09
  • Contact: Qi MA,Chuan DONG
  • About
  • Supported by:
    the Natural Science Foundation of Shanxi Province(201901D111309);the Research Project Foundation of Datong City(2019027)


Ammonium citrate dibasic, trometamol and neodymium nitrate are employed as raw materials to effectively synthesize blue fluorescent neodymium and nitrogen co-doped carbon dots (Nd,N-CDs) with a fluorescence quantum yield of up to 93.05% via hydrothermal method. The morphology and surface structure of Nd,N-CDs are characterized in detail by several techniques including transmission electron microscopy (TEM), powder X-ray diffraction (XRD), infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). In addition, ultraviolet visible absorption spectroscopy and fluorescence spectroscopy are used to investigate its optical properties and optical stability. Results show that drug molecule sulfasalazine (SSZ) could dramatic decrease the fluorescence of Nd,N-CDs, but other four drug molecules and metal ions have no significant influence. Thus, a fluorescence detection method for selective detection of SSZ is established. The linear detection range is between 0.1 μmol/L and 50 μmol/L, and the detection limit is 0.05 μmol/L. Further investigation shows that the fluorescence quenching mainly involves two mechanisms that are dynamic quenching and fluorescence resonance energy transfer. The as-established analytical method could be used to determine the content of SSZ in actual tablets with recovery ranging from 98.1%~104.2%. Furthermore, the synthesized Nd,N-CDs present good biocompatibility and could be used as a fluorescent probe to penetrate the cell wall to stain the cytoplasm, however, it could not enter the cell nucleus, which effectively avoids further cell damage.

Key words: Carbon dots, Sulfasalazine, Fluorescence analysis, Cell imaging

CLC Number: