Neodymium and Nitrogen Co‑doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging

doi:10.19894/j.issn.1000-0518.220041

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (11): 1726-1734.DOI: 10.19894/j.issn.1000-0518.220041

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Neodymium and Nitrogen Co‑doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging

Jin-Ping SONG¹, Qi MA¹(), Xiao-Min LIANG², Jian-Peng SHANG¹, Chuan DONG()

^1.College of Chemistry and Chemical Engineering，Institute of Applied Chemistry，Shanxi Datong University，Datong 037009，China
^2.School of Chemistry and Material Science，Shanxi Normal University，Linfen 041004，China
^3.Institute for Environmental Science，Shanxi University，Taiyuan 030006，China

Received:2022-02-21 Accepted:2022-04-20 Published:2022-11-01 Online:2022-11-09
Contact: Qi MA,Chuan DONG
About author:dc@sxu.edu.cn
maqihx@163.com；
Supported by:
the Natural Science Foundation of Shanxi Province(201901D111309);the Research Project Foundation of Datong City(2019027)

1. Neodymium and Nitrogen Co-doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging.pdf(186KB)

Abstract

Abstract:

Ammonium citrate dibasic， trometamol and neodymium nitrate are employed as raw materials to effectively synthesize blue fluorescent neodymium and nitrogen co-doped carbon dots （Nd，N-CDs） with a fluorescence quantum yield of up to 93.05% via hydrothermal method. The morphology and surface structure of Nd，N-CDs are characterized in detail by several techniques including transmission electron microscopy （TEM）， powder X-ray diffraction （XRD）， infrared spectroscopy （FT-IR） and X-ray photoelectron spectroscopy （XPS）. In addition， ultraviolet visible absorption spectroscopy and fluorescence spectroscopy are used to investigate its optical properties and optical stability. Results show that drug molecule sulfasalazine （SSZ） could dramatic decrease the fluorescence of Nd，N-CDs， but other four drug molecules and metal ions have no significant influence. Thus， a fluorescence detection method for selective detection of SSZ is established. The linear detection range is between 0.1 μmol/L and 50 μmol/L， and the detection limit is 0.05 μmol/L. Further investigation shows that the fluorescence quenching mainly involves two mechanisms that are dynamic quenching and fluorescence resonance energy transfer. The as-established analytical method could be used to determine the content of SSZ in actual tablets with recovery ranging from 98.1%~104.2%. Furthermore， the synthesized Nd，N-CDs present good biocompatibility and could be used as a fluorescent probe to penetrate the cell wall to stain the cytoplasm， however， it could not enter the cell nucleus， which effectively avoids further cell damage.

Key words: Carbon dots, Sulfasalazine, Fluorescence analysis, Cell imaging

CLC Number:

O657.3

Jin-Ping SONG, Qi MA, Xiao-Min LIANG, Jian-Peng SHANG, Chuan DONG. Neodymium and Nitrogen Co‑doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging[J]. Chinese Journal of Applied Chemistry, 2022, 39(11): 1726-1734.

Figures/Tables 9

Fig.1 Optimization of synthesis conditions of Nd，N-CDs

Fig.2 TEM image （A）， XRD （B）， FT-IR （C）， high resolution N1s （D） and C1s （E） spectra， and XPS survey spectrum （F） of Nd，N-CDs

Fig.3 （A） UV-Vis and fluorescence spectra of Nd，N-CDs；（B） Fluorescence spectra under different excitation wavelength；（C） Influence of pH on fluorescence intensity；（D） Influence of ionic strength on fluorescence intensity

Fig.4 （A） Fluorescence spectra before and after adding SSZ；（B） Selective response histogram；（C） Influence of pH on the detection of SSZ；（D） Linear curve of the detection of SSZB： The concentration of metal ions and drug molecular is 50 μmol/L， respectively

Table 1 Comparison of analytical methods for detection of SSZ

检测方法 Detection method	传感体系 Sensor system	线性范围 Linear range/（μmol·L^-1）	检出限 Detection limit/（μmol·L^-1）	参考文献 Ref.
色谱法 Chromatography	—	62.75 ~ 753	—	［2］
色谱法 Chromatography	—	0.5 ~ 7.5/7.5 ~125.5	0.5	［16］
比色法 Colorimetric method	Android smartphone	25 ~ 125	8.28	［4］
电化学法 Electrochemical method	NiO/CNT/IL/CPE	0.5 ~ 800	0.09	［3］
	MWCNTCOOH/BA?SPCE	1.0 ~ 14， 14 ~70	0.3	［17］
	BiNP?CNT/GCE	0.05 ~ 10	0.13	［18］
荧光法 Fluorescence method	N?CDs	0.85 ~ 17	0.08	［19］
	Tb?DPA complex	0.08 ~ 6， 1 ~100	0.06	［20］
	CdS nanoparticle	1.26 ~ 250	0.25	［21］
	Nd，N?CDs	0.1 ~ 50	0.05	本文 This work

Table 2 Stern?Volmer quenching constant and bimolecular reaction rate constant at different temperatures

温度

Temperature/K

猝灭常数

Quenching constant/（L·mol^-1）

双分子反应速率常数

Bimolecular reaction rate constant/（L·mol^-1·s^-1）

References 21

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样品 Sample	测定值 Found/（μmol·L^-1）	加标量 Spiked/（μmol·L^-1）	总测定值 Total found/（μmol·L^-1）	回收率 Recovery/%	相对标准偏差 RSD/%
1	8.18	0	8.18	—	3.2
2	8.18	5.00	13.14	99.2	4.4
3	8.18	10.00	17.99	98.1	7.0
4	8.18	15.00	23.81	104.2	2.7
5	8.18	25.00	32.82	98.6	0.6

样品 Sample	测定值 Found/（μmol·L^-1）	加标量 Spiked/（μmol·L^-1）	总测定值 Total found/（μmol·L^-1）	回收率 Recovery/%	相对标准偏差 RSD/%
1	8.18	0	8.18	—	3.2
2	8.18	5.00	13.14	99.2	4.4
3	8.18	10.00	17.99	98.1	7.0
4	8.18	15.00	23.81	104.2	2.7
5	8.18	25.00	32.82	98.6	0.6

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Neodymium and Nitrogen Co‑doped Carbon Dots with High Fluorescence Quantum Yield for Detection of Sulfasalazine and Hela Cell Imaging

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