Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

doi:10.11944/j.issn.1000-0518.2018.04.170463

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (4): 401-409.DOI: 10.11944/j.issn.1000-0518.2018.04.170463

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Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

CHEN Fan,GUO Zhiqian(),ZHU Weihong

Institute of Fine Chemicals,East China University of Science & Technology,Shanghai 200237,China

Received:2017-12-21 Accepted:2017-01-24 Published:2018-03-30 Online:2018-04-02
Contact: GUO Zhiqian
Supported by:
Supported by the National Natural Science Foundation of China(No.21622602, No.21325625),

Abstract

Abstract:

A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM) was synthesized from naphthalimide(NI), N-isopropyl acrylamide(NIPAM) and polyhedral oligomeric silsesquioxane(POSS) as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT). The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM) were characterized by nuclear magnetic resonance spectroscopy(NMR), fluorescence emission spectrometer and mass spectrometry(MS). The results show that Poly(POSS-NI-NIPAM) shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance. The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM) as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.

Key words: polymeric fluorescent probe, pH sensitivity, temperature sensitivity, polyhedral oligomeric silsesquioxane

CHEN Fan, GUO Zhiqian, ZHU Weihong. Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe[J]. Chinese Journal of Applied Chemistry, 2018, 35(4): 401-409.

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Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

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