Synthesis and Properties of Red-Emitting Fluorescence Probe for Viscosity Detection

doi:10.11944/j.issn.1000-0518.2020.04.190284

Chinese Journal of Applied Chemistry ›› 2020, Vol. 37 ›› Issue (4): 440-446.DOI: 10.11944/j.issn.1000-0518.2020.04.190284

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Synthesis and Properties of Red-Emitting Fluorescence Probe for Viscosity Detection

CAI Fengze,XU Yongling,ZHOU Le,XU Bingsong,CHEN Hao,SUN Jianqiang,LI Di,WANG Hui()

Department of Medical Biology,Wannan Medical College,Wuhu,Anhui 241002,China

Received:2019-10-24 Accepted:2019-12-25 Published:2020-04-01 Online:2020-04-10
Contact: WANG Hui
Supported by:
Supported by the Student Research Program of Wannan Medical College(No.WK2019S44), and Anhui Provincial Natural Science Foundation(No.1908085QB50)

Abstract

Abstract:

A red-emitting D-π-A type fluorescence probe (E)-3-(2-ethoxy-2-oxoethyl)-2-(4-((4-(methoxycarbonyl)phenyl)(phenyl)amino)styryl)benzo[d]thiazol-3-ium hexafluorophosphate salt(L) based on triphenylamine and benzothiazole salts for detecting viscosity was designed and synthesized, which was fully characterized by modern analytical testing methods. The systematic investigations indicated that probe L displayed a strong fluorescence emission at about 630 nm in viscous media, which could minimize the background noises effectively and improve the signal-to-noise(S/N) ratio of biological imaging. Probe L exhibited great sensitivity to viscosity. Moreover, probe L showed a good linear relationship (R²=0.9934) between the fluorescence intensity response (log (I/I₀)) and viscosity (log η). Besides, probe L exhibited a good selectivity and could serve as a fluorescent probe for sensing viscosity without being interfered by polarity and other analytes. Furthermore, biological experimental indicated that probe L possesses low cytotoxicity, which can be applied to the fluorescence imaging of intracellular microenvironment viscosity.

Key words: triphenylamine, fluorescent probe, viscosity, red emitting

CAI Fengze, XU Yongling, ZHOU Le, XU Bingsong, CHEN Hao, SUN Jianqiang, LI Di, WANG Hui. Synthesis and Properties of Red-Emitting Fluorescence Probe for Viscosity Detection[J]. Chinese Journal of Applied Chemistry, 2020, 37(4): 440-446.

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Synthesis and Properties of Red-Emitting Fluorescence Probe for Viscosity Detection

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