Preparation and Characterization of Graphene Oxide-Hyaluronic Acid-Polyethylene Glycol Composite Supramolecular Hydrogel

doi:10.19894/j.issn.1000-0518.230208

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (12): 1672-1681.DOI: 10.19894/j.issn.1000-0518.230208

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Preparation and Characterization of Graphene Oxide-Hyaluronic Acid-Polyethylene Glycol Composite Supramolecular Hydrogel

Wei-Na HAO¹, Chao ZHOU², Hai-Ping DI³, Lin-Hong DENG²()

^1.School of Pharmacy & School of Biological and Food Engineering，Changzhou University，Changzhou 213164，China
^2.School of Medical and Health Engineering，Changzhou University，Changzhou 213164，China
^3.Department of Burns，Zhengzhou First People's Hospital，Zhengzhou 450004，China

Received:2023-07-19 Accepted:2023-10-23 Published:2023-12-01 Online:2024-01-03
Contact: Lin-Hong DENG
About author:dlh@cczu.edu.cn
Supported by:
the Open Project of State Key Laboratory of Supramolecular Structure and Materials(sklssm2022037);Henan Medical Science and Technology Research Program （Joint Construction） Project(LHGJ20191000)

Abstract

Abstract:

Supramolecular hydrogels as biomaterials have been widely investigated for medical applications. However， the inadequate strength and stability of supramolecular hydrogels limit their applications in dressings， stents and drug carriers. In order to improve the mechanical properties of supramolecular hydrogels， a graphene oxide-hyaluronic acid-polyethylene glycol supramolecular hydrogel （GHCAP） is successfully prepared by the host-guest interaction of cyclodextrin and adamantane functional groups， with hyaluronic acid grafted cyclodextrin and graphene oxide （GO） as the main polymers and four-arm polyethylene glycol grafted adamantane as guest polymers. The elastic modulus of GHCAP-functionalized hydrogel is significantly increased compared with that of hyaluronic acid-polyethylene glycol supramolecular hydrogel （HCAP） without graphene oxide， and the loading rate of GHCAP-functionalized hydrogel for cichoric acid drugs reached 43.38% under the same conditions. Hence， the supramolecular hydrogels of GHCAP are expected to have wide application in the fields of medical biomaterials such as tissue dressings and drug carriers.

Key words: Supramolecular hydrogels, Graphene oxide, Hyaluronic acid, Drug release, Biocompatibility

CLC Number:

O636.1

Wei-Na HAO, Chao ZHOU, Hai-Ping DI, Lin-Hong DENG. Preparation and Characterization of Graphene Oxide-Hyaluronic Acid-Polyethylene Glycol Composite Supramolecular Hydrogel[J]. Chinese Journal of Applied Chemistry, 2023, 40(12): 1672-1681.

Figures/Tables 9

Fig.1 1H NMR spectra of CD-Tos（A）， CD-HDA（B）， CD-HA（C） and PEG-AD（D）

Fig.2 FT-IR spectra of （A） HA and modified hyaluronic acid （a.HA； b.CD-HA； c.CD-HA-GO）and （B） PEG and PEG-AD （a.PEG； b.PEG-AD）

Fig.3 XPS spectra of CD-HA-GO

Fig.4 SEM images of freeze-drying HCAP （A）， GHCAP （B）， DHCAP （C） and DGHCAP （D） supramolecular hydrogels at different magnificationsNote： Cyclodextrin-adamantane host-guest supramolecular hydrogel （HCAP）； Cyclodextrin-adamantane host-guest supramolecular hydrogel containing 2% graphene oxide （GHCAP）； Cichoric acid-loaded cyclodextrin-adamantane supramolecular hydrogel （DHCAP）； Cichoric acid-loaded cyclodextrin-adamantane host-guest supramolecular hydrogel containing 2% graphene oxide （DGHCAP）

Fig.5 TGA curves of HCAP （A） and GHCAP （B） supramolecular hydrogels

Fig.6 Rheological properties of HCAP （A， C） and GHCAP （B， D） supramolecular hydrogels：（A， B） Frequency sweep at strain of 0.1%；（C， D） Stress sweep at frequency of 1 Hz

Fig.7 Drug loading and release of HCAP and GHCAP supramolecular hydrogelsNote： A. The loading efficiency of HCAP and GHCAP； B. Cumulatively released drug amount； C. Drug amount released at each time point from HCAP and GHCAP

Fig.8 CCK-8 results of 24 h cell culture in vitro with different concentrations of HCAP and GHCAP

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Preparation and Characterization of Graphene Oxide-Hyaluronic Acid-Polyethylene Glycol Composite Supramolecular Hydrogel

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