Preparation and Properties of Mussel-Inspired Antibacterial Bone Adhesive

doi:10.19894/j.issn.1000-0518.230045

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (9): 1258-1266.DOI: 10.19894/j.issn.1000-0518.230045

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Preparation and Properties of Mussel-Inspired Antibacterial Bone Adhesive

Jia-Hui LIU¹^,², Bai-Chao AN³^,⁴, Qiu-Yan YAN¹(), Shi-Fang LUAN¹^,²

^1.State Key Laboratory of Polymer Physics and Chemistry，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022，China
^2.School of Applied Chemistry and Engineering，University of Science and Technology of China，Hefei 230026，China
^3.School of Pharmacy，Guangdong Medical University，Dongguan 523000，China
^4.School of Traditional Chinese Medicine，Southern Medical University，Guangzhou 510000，China

Received:2023-03-03 Accepted:2023-07-12 Published:2023-09-01 Online:2023-09-14
Contact: Qiu-Yan YAN
About author:qyyan@ciac.ac.cn
Supported by:
the National Natural Science Foundation of China(22107099);the National Key Research and Development Program of China(2021YFC2101700);Natural Science Foundation of Guangdong Province of China(2018A030313355);the High-Tech Research & Development Program of CAS-WEGO Group

Abstract

Abstract:

As one of the most commonly occurred trauma conditions， bone fractures have harmed the health of tens of millions of people. Bone adhesives have attracted wide attention due to the excellent adhesive property， however， poor biocompatibility may become a barrier hindering fracture healing， which restricts the wide use of bone adhesives. Inspired by mussel， a novel antibacterial bone adhesive is fabricated by blending the oxidized hyaluronic acid with tannic （TA） and poly-ε-lysine （PL）. Due to the electrostatic interaction and dynamic covalent bond， the bone adhesive exhibits prominent adhesive， self-healing and injectable prosperities， which could meet the different needs of clinical applications. The great cytocompatibility of the adhesive could offer an environment suitable for bone tissue healing. Moreover， the antibacterial activity of the adhesive that provided by TA could decrease the risk of bacterial infection at the site of fracture.

Key words: Dual-network bone adhesive, Mussel-inspired, Injectability, Biocompatibility, Antibacterial activity

CLC Number:

O636

Jia-Hui LIU, Bai-Chao AN, Qiu-Yan YAN, Shi-Fang LUAN. Preparation and Properties of Mussel-Inspired Antibacterial Bone Adhesive[J]. Chinese Journal of Applied Chemistry, 2023, 40(9): 1258-1266.

Figures/Tables 6

Fig.1 （A） Schematic illustration of fabrication of bone adhesive；（B） Schematic illustration of the synthesis of OHA；（C） FT-IR spectra of HA and OHA；（D） 1H NMR spectra of HA and OHA

Fig.2 （A） FT-IR spectra of OHA@PL；（B） Gelation process monitoring of OHA@PL@TA by rheological analysis；（C） Amplitude sweep results of OHA@PL@TA with different mass fraction of TA；（D） Strain sweep results of OHA@PL@TA；（E） Oscillation strain sweep of OHA@PL@TA followed by time sweep with high strains and low strains；（F） Viscosity change of OHA@PL@TA with the increase of shear rate

Fig.3 （A） Experiment set-up of lap-shear adhesion strength test. The lap-shear adhesion strength of OHA@PL@TA with different of the concentration of TA， when the concentration of PL respectively was 3%（B）， 4%（C）， 5%（D）， 6%（E） and 7%（F）（***represents P＜0. 001，ns represents P＞0.05， n=3）

Fig.4 （A） Experiment set-up of end-to-end adhesion strength test. The end-to-end adhesion strength of OHA@PL@TA with different of the concentration of TA， when the concentration of PL respectively was 3%（B）， 4%（C）， 5%（D）， 6%（E） and 7%（F）（*represents P＜0.05，***represents P＜0.001，ns represents P＞0.05， n=3）

Fig.5 （A） Images of survival S.aureus clones on the culture plate after being co-cultured with samples；（B） Images of survival E.coil clones on the culture plate after being co-cultured with samples；（C） S.aureus and （D） E.coli with filtering papers incubated with leach liquor of samples placed on the agar petri dish to analyse the inhibition zone （Zone 1， 2， 3 and 4 stand for control， OHA， OHA@PL and OHA@PL@TA group， respectively）. Quantitative results of S.aureus（E） and E.coli（F） co-cultured with PBS and those samples， respectively （***represents P＜0.001， n=3）

Fig.6 （A） Live/Dead staining of MC-3T3 after treating with samples for 24 h；（B） Cell viability of MC-3T3 treated with samples for 24 h （ns represents P＞0.05， n=3）

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Preparation and Properties of Mussel-Inspired Antibacterial Bone Adhesive

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