Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (4): 536-545.DOI: 10.19894/j.issn.1000-0518.220253

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Preparation and Properties of 3D Printed nHA/PEEK-AgNPs Composite Porous Scaffolds

Yu-Jie MA1, Ying-Xin ZHANG2, Huan-Yan DAI1, Zhi-Min XU1, Bing HAN1()   

  1. 1.Department of Oral and Maxillofacial Surgery II,Stomatological Hospital of Jilin University,Changchun 130021,China
    2.Department of Oral Emergency,Stomatological Hospital of Jilin University,Changchun 130021,China
  • Received:2022-07-26 Accepted:2023-02-22 Published:2023-04-01 Online:2023-04-17
  • Contact: Bing HAN
  • About
  • Supported by:
    the International Science and Technology Cooperation Project of Jilin Provincial Science and Technology Department(20190701035GH);the Industrial Technology and Research Project of Jilin Provincial Development and Reform Commission(2019C051-11)


Nanometer hydroxyapatite (nHA) and poly(ether-ether-ketone) (PEEK) powder is intensively mixed to develop and prepare nHA/PEEK-AgNPs composite porous scaffolds by fused deposition modeling (FDM) 3D printing technology. Then, dopamine oxidation polymerization and silver nitrate chemical reduction were used to form silver nanoparticle coating on the surface to prepare nHA/PEEK-AgNPs composite porous scaffolds. The scaffold had a unique three-dimensional porous structure with a surface contact angle of about (33.2±3.65)(°), showing good hydrophilicity. Mechanical test results showed that the maximum compressive strength of nHA/PEEK-AgNPs porous scaffolds was (47.4±3.9) MPa, which was significantly higher than (36.3±7.3) MPa of PEEK group. At the same time, there was no significant difference in the maximum elastic modulus between the PEEK group and the PEEK group, indicating that the mechanical strength of the composite was enhanced compared with that of the PEEK porous scaffold. The antibacterial test showed that the antibacterial rate of Escherichia coli and Staphylococcus aureus was (89.4±2.4)% and (85.8±4.4)%, respectively. The results of cell proliferation assay showed that the nHA/PEEK-AgNPs group had significantly better cell proliferation than the PEEK group at all time points within 7 days (P<0.05). In addition, nHA/PEEK-AgNPs had good cell compatibility and osteogenic activity. The results of RT-PCR showed that the expression levels of Runx2 gene and Col1 gene in nHA/PEEK-AgNPs group were up-regulated over time compared with PEEK group within 14 days (P<0.05). These results indicated that nHA/PEEK-AgNPs composite scaffolds could significantly improve the expression of osteogenesis-related genes. nHA/PEEK-AgNPs three-dimensional porous scaffolds are expected to be applied in the field of bone tissue engineering and repair.

Key words: 3D printing, nHA/PEEK-AgNPs, Three-dimensional porous scaffold, Antibacterial, Biocompatibility, Osteogenesis

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