Preparation and Properties of 3D Printed nHA/PEEK-AgNPs Composite Porous Scaffolds

doi:10.19894/j.issn.1000-0518.220253

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (4): 536-545.DOI: 10.19894/j.issn.1000-0518.220253

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Preparation and Properties of 3D Printed nHA/PEEK-AgNPs Composite Porous Scaffolds

Yu-Jie MA¹, Ying-Xin ZHANG², Huan-Yan DAI¹, Zhi-Min XU¹, Bing HAN¹()

^1.Department of Oral and Maxillofacial Surgery II，Stomatological Hospital of Jilin University，Changchun 130021，China
^2.Department of Oral Emergency，Stomatological Hospital of Jilin University，Changchun 130021，China

Received:2022-07-26 Accepted:2023-02-22 Published:2023-04-01 Online:2023-04-17
Contact: Bing HAN
About author:hbing@jlu.edu.cn
Supported by:
the International Science and Technology Cooperation Project of Jilin Provincial Science and Technology Department(20190701035GH);the Industrial Technology and Research Project of Jilin Provincial Development and Reform Commission(2019C051-11)

Abstract

Abstract:

Nanometer hydroxyapatite （nHA） and poly（ether-ether-ketone）（PEEK） powder is intensively mixed to develop and prepare nHA/PEEK-AgNPs composite porous scaffolds by fused deposition modeling （FDM） 3D printing technology. Then， dopamine oxidation polymerization and silver nitrate chemical reduction were used to form silver nanoparticle coating on the surface to prepare nHA/PEEK-AgNPs composite porous scaffolds. The scaffold had a unique three-dimensional porous structure with a surface contact angle of about （33.2±3.65）（°）， showing good hydrophilicity. Mechanical test results showed that the maximum compressive strength of nHA/PEEK-AgNPs porous scaffolds was （47.4±3.9） MPa， which was significantly higher than （36.3±7.3） MPa of PEEK group. At the same time， there was no significant difference in the maximum elastic modulus between the PEEK group and the PEEK group， indicating that the mechanical strength of the composite was enhanced compared with that of the PEEK porous scaffold. The antibacterial test showed that the antibacterial rate of Escherichia coli and Staphylococcus aureus was （89.4±2.4）% and （85.8±4.4）%， respectively. The results of cell proliferation assay showed that the nHA/PEEK-AgNPs group had significantly better cell proliferation than the PEEK group at all time points within 7 days （P＜0.05）. In addition， nHA/PEEK-AgNPs had good cell compatibility and osteogenic activity. The results of RT-PCR showed that the expression levels of Runx2 gene and Col1 gene in nHA/PEEK-AgNPs group were up-regulated over time compared with PEEK group within 14 days （P＜0.05）. These results indicated that nHA/PEEK-AgNPs composite scaffolds could significantly improve the expression of osteogenesis-related genes. nHA/PEEK-AgNPs three-dimensional porous scaffolds are expected to be applied in the field of bone tissue engineering and repair.

Key words: 3D printing, nHA/PEEK-AgNPs, Three-dimensional porous scaffold, Antibacterial, Biocompatibility, Osteogenesis

CLC Number:

O632

Yu-Jie MA, Ying-Xin ZHANG, Huan-Yan DAI, Zhi-Min XU, Bing HAN. Preparation and Properties of 3D Printed nHA/PEEK-AgNPs Composite Porous Scaffolds[J]. Chinese Journal of Applied Chemistry, 2023, 40(4): 536-545.

Figures/Tables 11

Table 1 Primer sequences

Gene	Forward primer sequence （5′-3′）	Reverse primer sequence （3′-5′）
GAPDH	AACTTTGGCATTGTGGAAGG	ACACATTGGGGGTAGGAACA
Runx2	GCCGGGAATGATGAGAACTA	GGACCGTCCACTGTCACTTT
Collagen1	CACAGAGGTTTCAGTGGTTTGG	GCACCAGTAGCACCATCATTTC

Fig.1 Appearance of material （A） PEEK，（B） nHA/PEEK，（C） nHA/ PEEK-AgNPs， and ESEM pictures of the microstructure and surface morphology of the material；（D） PEEK，（E） nHA/PEEK and （F） nHA/ PEEK-AgNPs were low magnification images；（G） PEEK，（H） nHA/PEEK and （I） nHA/ PEEK-AgNPs are high magnification images

Fig.2 FT-IR spectra of scaffold materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs）

Fig.3 XRD spectra of scaffold materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs）

Fig.4 Stress-strain curves （A） and comparison of maximum compressive strength （B） and maximum elastic modulus （C） of three groups of scaffold materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs）. n=3， *P＜0.05

Fig.5 Surface contact angles of three kinds of scaffold materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs）. n=3， *P＜0.05

Fig.6 The bacteriostasis rate of scaffold materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs） against Escherichia coli （B） and Staphylococcus aureus （C）. n=3， *P＜0.05

Fig.7 ESEM observation of surface cell adhesion spread on （A） PEEK，（B） nHA/PEEK and （C） nHA/PEEK-AgNPs

Fig.8 Cell proliferation of MC-3T3-E1 cells on materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs） at different time points （1， 3 and 7 d）. n=3， *P＜0.05

Fig.9 Relative alkaline phosphatase activity of MC-3T3-E1 cells on PEEK， nHA/PEEK and nHA/PEEK-AgNPs at different time points （3， 7 and 14 d）. n=3， *P＜0.05

Fig.10 Expression of osteogenic genes （Runx2， Col1） in MC-3T3-E1 cells on materials （PEEK， nHA/PEEK and nHA/PEEK-AgNPs） at different time points （3， 7 and 14 d）. n=3， *P＜0.05

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Preparation and Properties of 3D Printed nHA/PEEK-AgNPs Composite Porous Scaffolds

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