Preparation of Formyl Deoxycholic Acid Modified Fe3O4 Nanoparticles and Their Application for Oxytetracycline Loading

doi:10.19894/j.issn.1000-0518.220149

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (12): 1920-1926.DOI: 10.19894/j.issn.1000-0518.220149

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Preparation of Formyl Deoxycholic Acid Modified Fe₃O₄ Nanoparticles and Their Application for Oxytetracycline Loading

Tao GONG¹^,², Chao-Chao WEN², Kai-Li QIN¹, Ying-Zhu ZAHO¹, Yu-Qi ZHANG¹, Wen-Ting LIANG²(), Chuan DONG²()

^1.Department of Biochemistry and Molecular Biology，Shanxi Medical University，Taiyuan 030001，China
^2.Institute of Environmental Science，Shanxi University，Taiyuan 030006，China

Received:2022-04-22 Accepted:2022-07-27 Published:2022-12-01 Online:2022-12-13
Contact: Wen-Ting LIANG,Chuan DONG
About author:dc@sxu.edu.cn
liangwt@sxu.edu.cn
Supported by:
the National Natural Science Foundation of China(21976113);amd the Natural Science Foundation of Shanxi Province(202103021221240)

Abstract

Abstract:

Formyldeoxycholic acid modified Fe₃O₄ nanoparticles （FDCA-MNPs） have been successfully constructed. The structure， morphology and magnetic properties of FDCA-MNPs are characterized by Fourier transform infrared spectroscopy （FT-IR）， transmission electron microscopy （TEM） and vibrating sample magnetometer. Oxytetracycline （OTC） is used as a model drug to evaluate the drug loading and release behaviors of FDCA-MNPs. The maximum loading of OTC is 59.7 mg/g， and the loading behavior follows Freundlich adsorption balance equations with multilayer adsorption．Furthermore， FDCA-MNPs@OTC shows a higher release rate at pH=9 （11.8%） than that of pH=7 （8.8%）. The bacteriostatic activities of FDCA-MNPs， OTC， FDCA-MNPs@OTC against E.coli are compared in vitro. The results show that FDCA-MNPs have no inhibition-activity for E.coli， meanwhile， FDCA-MNPs@OTC displays better sterilization ability than OTC at the same concentration. The minimum antibacterial concentration is 3 μg/mL， and the antibacterial rate reaches 97.8%. It is proved that the synergistic effect of drug carrier and drugs reveals good antibacterial properties and reduce the dosage of antibiotics.

Key words: Fe₃O₄ nanoparticles, Terramycin, Drug release

CLC Number:

O651

Tao GONG, Chao-Chao WEN, Kai-Li QIN, Ying-Zhu ZAHO, Yu-Qi ZHANG, Wen-Ting LIANG, Chuan DONG. Preparation of Formyl Deoxycholic Acid Modified Fe₃O₄ Nanoparticles and Their Application for Oxytetracycline Loading[J]. Chinese Journal of Applied Chemistry, 2022, 39(12): 1920-1926.

Figures/Tables 7

Fig.1 The synthetic route of FDCA-MNPs

Fig.2 FT-IR spectra of APTES-MNPs and FDCA-MNPs （A） and TEM micrographs of FDCA-MNPs （B）

Fig.3 （A） XRD patterns of FDCA-MNPs；（B） Magnetic hysteresis loops of APTES-MNPs and FDCA-MNPs；（C） TGA curves of APTES-MNPs and FDCA-MNPs

Fig.4 Adsorption isotherms of OTC on FDCA-MNPs （A） and fitting of adsorption isotherm data with Langmuir （B） and Freundlich （C）

Fig.5 Release profiles of OTC from FDCA-MNPs@OTC in different PBS （pH=7 and 9）

Fig.6 Images of bacteriostasis effect on E.coli by FDCA-MNPs（A）， OTC（B） and FDCA-MNPs@OTC（C）各组物质的质量浓度从左到右依次为1、3、5、7、11和20 μg/mL from left to right， the mass concentrations of each material are 1， 3， 5， 7， 11 and 20 μg/mL， respectively

Fig.7 Images of bacteriostasis effect on E.coli （5.0×108 CFU/mL） by magnetic nanoparticles and ρ（FDCA-MNPs@OTC）=0， 1， 3， 5， 7 and 11 μg/mL， respectively （from left to right）

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Preparation of Formyl Deoxycholic Acid Modified Fe₃O₄ Nanoparticles and Their Application for Oxytetracycline Loading

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