Study on Membrane Blocking in Lycium Barbarum Polysaccharide Ultrafiltration Membrane Bioreactor Based on COMSOL Software

doi:10.19894/j.issn.1000-0518.210535

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (12): 1912-1919.DOI: 10.19894/j.issn.1000-0518.210535

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Study on Membrane Blocking in Lycium Barbarum Polysaccharide Ultrafiltration Membrane Bioreactor Based on COMSOL Software

Bo WANG¹, Fang LUO¹, Yan-Zhao SUN²(), Jing-Wen HU¹, Jun-Miao LIU¹

^1.School of Pharmacy Engineering，Zhejiang Pharmaceutical University，Ningbo 315100，China
^2.School of Automotive and Traffic Engineering & Hubei University of Arts and Science，Xiangyang 441053，China

Received:2021-11-15 Accepted:2022-09-21 Published:2022-12-01 Online:2022-12-13
Contact: Yan-Zhao SUN
About author:sunyanzhao888@163.com
Supported by:
the General Scientific Research Project of Education Department of Zhejiang Province(Y202045027);the Natural Science Foundation of Ningbo City(2019A610371);the Universal-level Project of Zhejiang Medical College(2019011);the Science and Technology Innovation Program of College Students of Zhejiang Province(New Seedling Talent Program 2019R458004)

Abstract

Abstract:

Lycium barbarum polysaccharide is refined based on polysulfone hollow fiber ultrafiltration membrane separation technology， and the lycium barbarum polysaccharide membrane blocking countercurrent extraction and ultrasonic equipment parameters are corrected by COMSOL software to obtain a better purification process. A microwave-ultrasonic method is used to extract Lycium barbarum polysaccharides， and polysulfone hollow fiber ultrafiltration membranes are used to separate Lycium barbarum polysaccharides. Single factor analysis and orthogonal experiments are used to investigate the important parameters in the extraction and separation process of Lycium barbarum polysaccharides. The important membrane blocking parameters are corrected by the COMSOL software. The optimal process conditions for the extraction of Lycium barbarum polysaccharides are： the extraction temperature between 50~70 ℃， ultrasonic power 50 W， solid-liquid ratio 1∶8~1∶12， and extraction time 40~60 min. When the ultrafiltration membrane relative molecular weight cut-off is 1×10⁴， the membrane flux is better. The orthogonal experiment results show that the membrane flux has the largest relationship with the temperature of the feed liquid， followed by the membrane pH value and separation operating pressure. COMSOL software performs continuous countercurrent extraction equipment for Lycium barbarum polysaccharides and the calculations show that compared with fully enclosed blades， perforated blades can significantly reduce the solvent short-circuit phenomenon and increase the minimum flow rate by up to 65 times. The COMSOL software performs simulation calculations on the ultrasonic equipment. When the double ultrasonic transducers are axially separated by 12 cm， the axis crosses 90（°） and the power is set to 50 W， the regional mass transfer flux can be significantly increased. Based on the revision of COMSOL software data， the separation efficiency of Lycium barbarum polysaccharides has been greatly improved， which provides necessary data accumulation for the industrial production of Lycium barbarum polysaccharides in the future.

Key words: COMSOL software, Lycium barbarum polysaccharide, Ultrafiltration membrane, Bioreactor, Membrane obstruction

CLC Number:

O658

Bo WANG, Fang LUO, Yan-Zhao SUN, Jing-Wen HU, Jun-Miao LIU. Study on Membrane Blocking in Lycium Barbarum Polysaccharide Ultrafiltration Membrane Bioreactor Based on COMSOL Software[J]. Chinese Journal of Applied Chemistry, 2022, 39(12): 1912-1919.

Figures/Tables 12

Table 1 Setting of extraction process conditions of Lycium barbarum polysaccharides

固液比 Solid-liquid ratio/（mg∶mL）	提取温度 Extraction temperature/℃	提取时间 Extraction time/min	超声功率 Ultrasonic power/W
1∶6	20	10	10
1∶8	40	20	15
1∶10	60	30	20
1∶12	80	40	25
-	-	50	30
-	-	60	35
-	-	-	40
-	-	-	45
-	-	-	50

Fig.1 The technological process of wolfberry polysaccharide ultrafiltration membrane

Table 2 Influencing factors and levels of ultrafiltration membrane separation of Lycium barbarum polysaccharides

水平 Level	因素Factor
水平 Level	A温度Temperature/℃	B压力 Pressure/MPa	C酸碱度 pH
1	25	0.04	5.0
2	30	0.07	6.0
3	40	0.10	7.0

Fig.2 Effect of extraction temperature of Lycium barbarum polysaccharide on extraction yield

Fig.3 Effect of extraction time of Lycium barbarum polysaccharides on the extraction yield

Fig.4 Extraction power of Lycium barbarum polysaccharides

Fig.5 Solid-liquid ratio of Lycium barbarum polysaccharide extraction

Table 3 Separation characteristics of ultrafiltration membranes

截留相对分子质量Intercept relative molecular mass/kD

料液

Liquid

透过液

Permeate

截留率Retention rate/%

膜通量

Membrane flux/（mL·cm^-2·min^-1）

体积

Volume/L

多糖浓度

Polysaccharide concentration/（g·L^-1）

体积 Volume/L

多糖浓度

Polysaccharide concentration/（g·L^-1）

Table 4 Multi-factor levels of Lycium barbarum polysaccharide ultrafiltration membrane separation

因素 Factor		膜通量 Membrane flux/（mL·cm^-2·min^-1）	截留率 Retention rate/%
温度 Temperature/℃	25	0.019±0.001 2	85.28±0.41
	30	0.028±0.001 0	83.16±0.59
	40	0.035±0.001 7	80.23±0.26
压力Pressure/MPa	0.04	0.017±0.000 3	88.86±0.73
	0.07	0.021±0.000 8	86.94±0.85
	0.10	0.024±0.000 9	83.58±0.84
pH	5.0	0.019±0.000 5	85.68±1.57
	6.0	0.018±0.000 4	86.45±1.82
	7.0	0.019±0.000 8	76.03±1.81

Fig.6 Solute distribution on the surface of the ultrafiltration membranecm（mol/L） is the solute concentration on the surface of the ultrafiltration membrane； cb（mol/L）is the solute concentration in the reactor； D is the diffusion coefficient； cp（mol/L） is the solute concentration on the side after passing through the ultrafiltration membrane module； JC（mL/（cm2·min）） is the membrane flux of the solute through the boundary layer； JCp（mL/（cm2·min）） is the solute flux through one side of the ultrafiltration membrane module

Table 5 Constant pressure filter clogging model

堵塞模型 Clogging model	滤饼层过滤模型 Filter cake layer filtration model	中间堵塞模型 Intermediate blockage model	标准堵塞模型 Standard clogging model	完全堵塞模型 Fully blocked model
数学模型 Mathematical model	1/J_p²=1/J₀²+K₁t/J₀	1/J_p=1/J₀+K₂t	1/J_p²=1/ $J 0 0.5$ +0.5 $J 0 0.5$ K₃t	1/J_p/1/J₀－K₄t
拟合方程 Fitting equation	y=K₁x+b	y=K₂x+b	y=0.5K₃x+b	y=－K₄x+b
y参数 y parameter	y=J₀/ $J p 2$	y=1/J_p	y=1/（J_PJ₀）	y/1/J_p
b参数 b parameter	b=1/J₀	b=1/J₀	b=1/J₀	b/1/J₀
x参数 x parameter	x=t	x=t	x=t	x=t

Table 5 Constant pressure filter clogging model

堵塞模型 Clogging model	滤饼层过滤模型 Filter cake layer filtration model	中间堵塞模型 Intermediate blockage model	标准堵塞模型 Standard clogging model	完全堵塞模型 Fully blocked model
数学模型 Mathematical model	1/J_p²=1/J₀²+K₁t/J₀	1/J_p=1/J₀+K₂t	1/J_p²=1/ $J 0 0.5$ +0.5 $J 0 0.5$ K₃t	1/J_p/1/J₀－K₄t
拟合方程 Fitting equation	y=K₁x+b	y=K₂x+b	y=0.5K₃x+b	y=－K₄x+b
y参数 y parameter	y=J₀/ $J p 2$	y=1/J_p	y=1/（J_PJ₀）	y/1/J_p
b参数 b parameter	b=1/J₀	b=1/J₀	b=1/J₀	b/1/J₀
x参数 x parameter	x=t	x=t	x=t	x=t

Fig.7 Streamline diagrams of fully enclosed blades and perforated bladesA. Fully enclosed blade streamline diagram； B. Perforated blade streamline diagram

References 13

1	谢文，陈华国，赵超，等. 枸杞多糖的生物活性及作用机制研究进展［J］. 食品科学， 2021， 42（5）： 349-359.
	XIE W， CHEN H G， ZHAO C， et al. Research progress on the biological activity and mechanism of Lycium barbarum polysaccharides［J］. Food Sci， 2021， 42（5）： 349-359.
2	王海彬，董志军，郭立涛，等. 枸杞多糖对糖尿病大鼠视网膜Caspase-3、Bcl-2和Bax表达的影响［J］. 中国老年学杂志， 2019， 39（20）： 5070-5074.
	WANG H B， DONG Z J， GUO L T， et al. Effects of Lycium barbarum polysaccharides on the expression of Caspase-3， Bcl-2 and Bax in the retina of diabetic rats［J］. Chinese J Gerontol， 2019， 39（20）： 5070-5074.
3	葛君，刘爱琴，黄文宇，等. 枸杞多糖联合美沙拉嗪治疗慢性实验性结肠炎小鼠模型的实验研究［J］. 中国中西医结合杂志， 2019， 39（11）： 1366-1371.
	GE J， LIU A Q， HUANG W Y， et al. Experimental study of Lycium barbarum polysaccharide combined with mesalazine in the treatment of chronic experimental colitis in a mouse model［J］. Chinese J Integr Tradition Chinese Western Med， 2019， 39（11）： 1366 -1371.
4	孙玉姣，高润凝，崔湘怡，等. 枸杞多糖及其硫酸化产物的免疫调节活性［J］. 陕西科技大学学报， 2020， 38（1）： 44-49.
	SUN Y J， GAO R N， CUI X Y， et al. Immunomodulatory activity of Lycium barbarum polysaccharide and its sulfated products［J］. J Shaanxi Univ Sci Technol， 2020， 38（1）： 44-49.
5	胡玲玲，乌雪燕. 枸杞多糖的水提醇沉法工艺优化［J］. 贵州农业科学， 2021， 49（7）： 128-133.
	HU L L， WU X Y. Process optimization of water extraction and alcohol precipitation of Lycium barbarum polysaccharide［J］. Guizhou Agric Sci， 2021， 49（7）： 128-133.
6	张倩，李书启. 不同提取方法对枸杞多糖提取率及抗氧化活性的影响［J］. 江苏农业科学， 2019， 47（3）： 169-173.
	ZHANG Q， LI S Q. The effect of different extraction methods on the extraction rate and antioxidant activity of Lycium barbarum polysaccharides［J］. Jiangsu Agric Sci， 2019， 47（3）： 169-173.
7	宫春宇，邢悦，单佳明，等. 玉米须多糖超滤制备工艺及成分分析［J］. 食品科技， 2020， 45（6）： 249-253.
	GONG C Y， XING Y， SHAN J M， et al. Ultrafiltration preparation process and composition analysis of corn silk polysaccharide［J］. Food Sci Technol， 2020， 45（6）： 249-253.
8	张婕，李云云，孙书培，等. 羟基红花黄色素A的提纯新工艺研究［J］. 河南化工， 2011， 6（28）： 39-42.
	ZHANG J， LI Y Y， SUN S P， et al. Study on the new purification process of Hydroxysafflor Yellow A［J］. Henan Chem Ind， 2011， 6（28）： 39-42.
9	叶红，周春宏，曾晓雄，等. 马尾藻多糖的膜分离纯化［J］. 湖北农业科学， 2011， 50（2）： 375-377.
	YE H， ZHOU C H， ZENG X X， et al. Membrane separation and purification of sargassum polysaccharides［J］. Hubei Agric Sci， 2011， 50（2）： 375-377.
10	张曜武，冯雪. 膜分离制备红松松塔多糖的研究［J］. 中国林副特产， 2013， 5： 18-20.
	ZHANG Y W， FENG X. Study on the preparation of Korean pine pine cone polysaccharide by membrane separation［J］. China Forest By-prod， 2013， 5： 18-20.
11	张涛，章伟睿，徐灿华，等. COMSOL与Visual C++三维电阻抗有限元联合建模与仿真研究［J］. 医疗卫生装备， 2017， 38（8）： 1-4.
	ZHANG T， ZHANG W R， XU C H， et al. COMSOL and Visual C++ three-dimensional electrical impedance finite element joint modeling and simulation research［J］. Med Health Equipm， 2017， 38（8）： 1-4.
12	焦会青，盛钰，赵成义，等. 基于COMSOL软件的绿洲盐渍化土壤中多离子耦合运移模型构建［J］. 农业工程学报， 2018， 34（15）： 100-106.
	JIAO H Q， SHENG Y， ZHAO C Y， et al. Construction of multi-ion coupled transport model in oasis salinized soil based on COMSOL software［J］. Transact Chinese Soc Agric Eng， 2018， 34（15）： 100-106.
13	温文娟，刘珊，黄远丽. 苯酚硫酸法与蒽酮硫酸法测定香菇多糖含量比较［J］. 现代食品，2020（21）： 177-179.
	WEN W J， LIU S， HUANG Y L. Comparison of determination of lentinan content by phenol-sulfuric acid method and anthrone-sulfuric acid method［J］. Modern Food， 2020（21）： 177-179.

[1]	HOU Shuhua, ZHENG Jifu, DONG Xue. Antifouling Properties of Poly(aryl ether ketone) Ultrafiltration Membranes by Surface-Grafting Amino Acids [J]. Chinese Journal of Applied Chemistry, 2017, 34(6): 644-648.
[2]	Yin Xiuli, Song Yanqiu. Ultrafiltration Membrane from PVDF/PAN Blend [J]. Chinese Journal of Applied Chemistry, 1997, 0(5): 112-114.
[3]	Xing Lanmin, Lu Fengcai. Study on Preparation and Characterization of Polyphenylquinoxaline Ultrafiltration Membrane Containing Biphenylene [J]. Chinese Journal of Applied Chemistry, 1994, 0(3): 16-20.
[4]	Wu Guanying, Sun Benhui, Guo lin, Shen Yibing, L? Suwei, Dai Weiguo. INFLUENCES OF THE SOLVENT EVAPORATION RATE ON THE PREPARATION OF PVC UF MEMBRANE [J]. Chinese Journal of Applied Chemistry, 1988, 0(5): 48-52.

Study on Membrane Blocking in Lycium Barbarum Polysaccharide Ultrafiltration Membrane Bioreactor Based on COMSOL Software

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