一种水溶性甲氧基萘乙烯半菁合成及对铬(Ⅲ)离子的荧光检测

doi:10.19894/j.issn.1000-0518.200372

摘要/Abstract

摘要： 设计合成了一种水溶性吲哚乙烯基半菁衍生物荧光探针D。实验证明,在纯水和4-羟乙基哌嗪乙磺酸(HEPES)缓冲溶液体系中,探针D可以对Cr³⁺进行裸眼识别。通过紫外-可见吸收光谱和荧光发射光谱,研究了探针D对Cr³⁺的具体识别性能。探针D对Cr³⁺识别不易受其它金属阳离子的干扰,探针D与Cr³⁺的配合比为1∶1,检测限为0.89×10^-8 mol/L,低于世界卫生组织(WHO)要求的饮用水中Cr³⁺浓度(9.60×10^-8 mol/L),说明探针D对Cr³⁺识别具有灵敏专一性的特点。使用荧光发射光谱检测不同水样中Cr³⁺的含量,证明探针D在实际条件下也可以对Cr³⁺进行定量分析,说明探针D具有可实际应用价值。

关键词: 荧光探针, 水溶性, 铬(Ⅲ)离子探针, 吲哚半菁, 甲氧基萘

Abstract: A water-soluble fluorescent probe D based on a derivative of indolevinyl hemicyanine was designed and synthesized. Experimental evidence shows that probe D could be used to identify Cr³⁺ with naked eyes in distilled water and 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer. The specific recognition performance of probe D for Cr³⁺ was determined by ultraviolet-visible (UV-Vis) absorption spectroscopy and fluorescence emission spectroscopy. The results prove that the recognition of probe D for Cr³⁺ will not be impacted by other metal cations, and the complexation ratio of probe D and Cr³⁺ is 1∶1. The detection limit is 0.89×10^-8 mol/L which is lower than the Cr³⁺ content (9.60×10^-8 mol/L) in the drinking water standard recommended by the World Health Organization (WHO), demonstrating the sensitivity and specificity of probe D for Cr³⁺. Fluorescence emission spectroscopy was used to detect Cr³⁺ content in different water samples. The testing results show that probe D could analyze Cr³⁺ quantitatively under actual conditions, indicating its potential in the practical application.

Key words: Fluorescent probe, Water soluble, Chromium(Ⅲ) ion probe, Indole hemicyanine, Methoxynaphthalene

中图分类号:

O657.3

黄译文, 王丽艳, 赵冰, 宋波. 一种水溶性甲氧基萘乙烯半菁合成及对铬(Ⅲ)离子的荧光检测[J]. 应用化学, 2021, 38(11): 1503-1511.

HUANG Yi-Wen, WANG Li-Yan, ZHAO Bing, SONG Bo. Synthesis of Water Soluble Methoxynaphthene Hemicine for Detection of Chromium(Ⅲ) Ion[J]. Chinese Journal of Applied Chemistry, 2021, 38(11): 1503-1511.

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