以香豆素为母体快速检测内源性甲醛的荧光探针

doi:10.11944/j.issn.1000-0518.2019.09.190023

摘要/Abstract

摘要：

基于甲醛(FA)与肼易发生亲核加成反应,从而阻断肼与荧光母体间电子传递的光诱导电荷转移(PET)机理,设计合成了以香豆素为母体的甲醛荧光探针3-苯并噻唑-7-肼香豆素(Coun)。实验结果显示,Coun与FA的最佳反应时间为4 min,极大地提高了检测效率,检测限为5×10^-6 μmol/L。该探针具有良好的细胞膜通透性,可应用于生物领域,检测细胞内的甲醛含量。

关键词: 荧光探针, 甲醛, 响应快, 细胞成像

Abstract:

A coumarin-based formaldehyde(FA) fluorescent probe Coun was designed and synthesized. The mechanism of this fluorescent probes for FA is based on blocking electron transfer between hydrazine and the dye upon FA nucleophilic addition. The results show that the optimal reaction time of Coun and FA is 4 min, which greatly improved the detection efficiency with detection limit of 5×10^-6 μmol/L. In addition, the probe has good cell membrane permeability, and can be used in the biological field to detect endogenous formaldehyde.

Key words: fluorescent probe, formaldehyde, fast response, cell imaging

高漫, 何鑫, 崔京南, 刘涛, 田镇豪, 何深贵. 以香豆素为母体快速检测内源性甲醛的荧光探针[J]. 应用化学, 2019, 36(9): 1053-1060.

GAO Man, HE Xin, CUI Jingnan, LIU Tao, TIAN Zhenhao, HE Shengui. A Coumarin-Based Fluorescent Probe for Rapid Detection of Endogenous Formaldehyde[J]. Chinese Journal of Applied Chemistry, 2019, 36(9): 1053-1060.

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