Chinese Journal of Applied Chemistry ›› 2010, Vol. 27 ›› Issue (04): 398-403.DOI: 10.3724/SP.J.1095.2010.90334

• Full Papers • Previous Articles     Next Articles

Synthesis and Cytotoxicity of N,N-Bis(8-flavonmethyl)geranylamine Derivatives

ZHAO Jin1, XIE Huan-Xu2, LIU Hong-Yu2, MA Hong-Xia2, XIE Song-Qiang3, WANG Chao-Jie1*   

  1. (1.Key Laboratory of Natural Medicine and Immune Engineering,Henan University,Kaifeng;
    2.College of Chemistry and Chemical Engineering,Henan University,Kaifeng;
    3.College of Pharmacy,Henan University,Kaifeng 475004)
  • Received:2009-05-08 Revised:2009-07-22 Published:2010-04-10 Online:2010-04-10


Four N,N-bis(8-flavonmethyl)geranylamine derivatives were designed and synthesized. The structures of the synthesized compounds were determined from the results of 1H NMR, MS and elemental analysis. The prelimilary cytotoxicity was evaluated on K562 and SMMC7721 cell lines by MTT assay. The results demonstrated that all compounds possessed antitumor actitity and compound 1c is the best among them with an IC50 value of 5.78 μmol/L or 3.85 μmol/L against K562 or SMMC7721 cell lines, respectively. The in vitro antitumor activities of compounds 1a and 1c against K562 were better than that of the commercial drug Melphalan. The interaction of compound 1c with herring sperm DNA was explored with ethidium bromide(EB) as a fluorescence probe. The result of the study on fluorescence quenching by DNAEB confirms the strong interaction of compound 1c with Herring Sperm DNA.

Key words: N,N-bis(flavonmethyl)geranylamine, synthesis, bioactivity, fluorescence

CLC Number: