Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (8): 1303-1311.DOI: 10.19894/j.issn.1000-0518.210399

• Full Papers • Previous Articles     Next Articles

A Visual Semi⁃quantitative Method for Rapid Detection of Copper Ion in Water

Feng-Zhou XU1,2,3(), Hua-Ying TANG1, Wu-Hui LIU1, Yi-Feng JIANG1, Wen-Kai LI1, Xian-Hai LU1   

  1. 1.College of Environmental and Biological Engineering,Putian University,Putian 351100,China
    2.Fujian Provincial Key Laboratory of Ecology-toxicological Effects & Control for Emerging Contaminants,Putian University,Putian 351100,China
    3.Key Laboratory of Ecological Environment and Information Atlas,Fujian Province University,Putian University,Putian 351100,China
  • Received:2021-08-09 Accepted:2021-11-15 Published:2022-08-01 Online:2022-08-04
  • Contact: Feng-Zhou XU
  • About
  • Supported by:
    the Natural Science Foundation of Fujian Province(2020J05209);the Education and Scientific Research Project for Young and Middle?aged Teachers of Fujian Province(JAT190569);the Scientific Research Start?up Project of Putian University(2019116);the University Students Innovation and Entrepreneurship Training Plan of Fujian(S202011498013)


Copper is one of the heavy metal pollutants. The development of point of care testing (POCT) is necessary in the emergency detection of pollution events, which can avoid the spread of the contaminated area. Based on DNA-stabilized fluorescent copper nanoparticles (CuNPs), a ultraviolet(UV)-assisted POCT (fluorescence colorimetric) was developed for detection of Cu2+. In this method, after optimization of conditions, CuNPs were synthesized in the MOPS solution using 600 nmol/L poly(AT-TA) dsDNA as the scaffold and 2 mmol/L sodium ascorbate as the reducing agent. With the aid of a UV flashlight, the red-orange fluorescence of CuNPs is visible to naked-eye (wearing anti-UV goggles). Also, the fluorescence brightness varies with the concentration of Cu2+. Based on this strategy, a semi-quantitative detection method for Cu2+ was developed, i.e., the concentration range of Cu2+ in the water-sample could be detected by fluorescent colorimetry with standard series. The LOD of the method is 1.5 mg/L (24 μmol/L), which meets the requirements of third-level of discharge standard in the Comprehensive Sewage Discharge Standard (GB 8978-1996). This method is a new semi-quantitative, visual point of care testing for Cu2+, which can provide a reference method for rapid on-site detection of copper pollution in environmental water.

Key words: Copper ions, Copper nanoparticles, Fluorescence colorimetry, Visualization

CLC Number: