Chinese Journal of Applied Chemistry ›› 2010, Vol. 27 ›› Issue (03): 363-366.DOI: 10.3724/SP.J.1095.2010.90180

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Glucose biosensor based on gold nanoparticles and enzyme embedded in silica sol-gel network

GAO Yan-Sheng, WANG Yuan, DI Jun-Wei*   

  1. (College of Chemistry and Chemical Engineering,Suzhou University,Suzhou 215123)
  • Received:2009-03-18 Revised:2009-06-02 Online:2010-03-10 Published:2010-03-10

Abstract:

A novel method for fabrication of glucose electrochemical biosensor based on simultaneously embedding gold nanoparticles and glucose oxidase in silica sol-gel network on gold electrode surface was investigated. The preparation conditions were optimized. The enzyme electrode displayed an excellent electrocatalytic response to glucose. The glucose sensor had a linear range from 0.02 to 2.0 mmol/L and the detection limit of 0.005 mmol/L. It was found that the response current to glucose decreased about 8% after storage at 4℃ for 100 days. The biosensor exhibited high sensitivity, rapid response and good long-term stability.

Key words: gold nanoparticles, sol-gel, glucose, electrochemical biosensor

CLC Number: