Separation and Purification of Baicalin Using Deep Eutectic Solvent and Surfactant

doi:10.19894/j.issn.1000-0518.210022

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (12): 1647-1654.DOI: 10.19894/j.issn.1000-0518.210022

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Separation and Purification of Baicalin Using Deep Eutectic Solvent and Surfactant

LI Ye-Hui, LI Xiao-Ning, HUI Wei, WANG Xiang, WANG Hai-Jun^*

School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China

Received:2021-01-30 Revised:2021-06-07 Published:2021-12-01 Online:2022-02-01
Supported by:
Postgraduate Research & Practice Innovation Program of Jiangsu Province (No.KYCX20_1777)

Abstract

Abstract: The deep eutectic solvents (DESs) are used to replace the traditional solvents and a new method is established for the separation of baicalin by the eutectic solvent-cloud point separation (DESs-CPE) system. Many variables including the type of DESs in the extractants, the water content in the DES, the solid-liquid ratio, the extraction temperature and time are evaluated and optimized. Two steps of cloud point separations are used to separate and recover baicalin to remove DESs. Quantitative and qualitative analysis of baicalin is carried out by UPLC. Under optimized experimental conditions, the maximum extraction efficiency of baicalin is (124.73±3.81) mg/g, about 1.64 times of the traditional method. After two cloud point separations, the maximum recovery rate of baicalin is (72.46±0.72)% and the purity is (89.72±1.14)%.

Key words: Baicalin, Deep eutectic solvent, Cloud point separation, Recovery, Purification

CLC Number:

O629.9

LI Ye-Hui, LI Xiao-Ning, HUI Wei, WANG Xiang, WANG Hai-Jun. Separation and Purification of Baicalin Using Deep Eutectic Solvent and Surfactant[J]. Chinese Journal of Applied Chemistry, 2021, 38(12): 1647-1654.

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Separation and Purification of Baicalin Using Deep Eutectic Solvent and Surfactant

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