Chinese Journal of Applied Chemistry ›› 2019, Vol. 36 ›› Issue (5): 585-594.DOI: 10.11944/j.issn.1000-0518.2019.05.180258

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Preparation of Molecularly Imprinted Polymers by Superoxide Dismutase-Catalyzed Electrochemically-Mediated Atom Transfer Radical Polymerization

LIU Yutong,ZHAO Mengyuan,LI Siyu,YANG Yifei,SUN Yue()   

  1. School of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian,Liaoning 116029,China
  • Received:2018-08-08 Accepted:2018-12-18 Online:2019-05-01 Published:2019-05-06
  • Contact: SUN Yue
  • Supported by:
    Supported by National Natural Science Foundation of China(No.21304041), the Liaoning Provincial Key Laboratory of Education(No.L201683656), the Dalian High-level Talent Innovation Support Project(No.2016RQ047)


Sensitive detection of metal-containing proteins is extremely important in pathology. Superoxide dismutase(SOD) was represented as metal-containing protein to prepare protein imprinted polymers(PIPs) and used for electrochemical biosensor. SOD was used as a template molecule and as a catalyst for electrochemically mediated atom transfer radical polymerization(eATRP) reaction to prepare SOD PIPs. This method does not require transition metal ions and owns the advantages of simple preparation, reagent saving, environmental protection, etc. In this experiment, we selected L-cysteine(L-cys) and nano gold modified gold electrode(Au/L-cys/nanoAu) as one working electrode for catalytic reduction of SOD, and initiator (4-mercaptophenyl 2-bromo-2-methylpropanoate, 4-HTP-Br) modified Au electrode as another working electrode for SOD PIPs modification. The electrode modified with PIPs was characterized by cyclic voltammetry(CV) and X ray photoelectron spectroscopy(XPS). Finally, the PIPs-modified electrode was used as a biosensor for the determination of SOD by differential pulse voltammetry(DPV) measurement. The linear range is 1.0×10-7 to 100 mg/L with the detection limit of 6.8×10-8 mg/L(S/N=3), the correlation coefficient is 0.995. Compared with other methods for detecting SOD, this method has a wider linear range and lower detection limits. This work is significant for the preparation of PIPs, eATRP catalyzed by proteins and the sensitive detection of metal-containing proteins.

Key words: protein imprinted polymer, electrochemically-mediated atom transfer radical polymerization, superoxide dismutase, sensor