Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (5): 736-748.DOI: 10.19894/j.issn.1000-0518.210144

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Advances in Construction and Electrochemical Biosensing of Antifouling Interfaces

Zhen-Hua LI1,3, Ying ZHU1,2, Jing CHEN1,2, Shi-Ping SONG1,2()   

  1. 1.Division of Physical Biology,CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China
    2.The Interdisciplinary Research Center,Shanghai Synchrotron Radiation Facility,Zhangjiang Laboratory,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China
    3.University of Chinese Academy of Sciences,Beijing 100049,China
  • Received:2021-03-25 Accepted:2021-06-23 Published:2022-05-01 Online:2022-05-24
  • Contact: Shi-Ping SONG
  • About
  • Supported by:
    the National Key R&D Program of China(2018YFF0212803);the National Natural Science Foundation of China(21974147)


Electrochemical biosensors have made rapid development in the field of wearable healthcare monitoring and have great potential applications in clinical detection due to high sensitivity, good portability, fast response and easy integration. However, non-specific adsorption of non-target biological substances on the electrode surface (i.e., biofouling) when exposed to the actual clinical biological sample affects the performance of electrochemical biosensors. Therefore, the construction of a sensing interface with antifouling capability (antifouling interface) to prevent non-target substances from adsorbing to the electrode surface is of great importance for expanding the practical application range of electrochemical sensors and realizing detection in complex biological samples. In this review, antifouling electrode interfaces based on physical, chemical, or biological strategies and their application in clinical biomarker detection are summarized, which provides technical references for the improvement of electrochemical biosensors in practical application. Finally, we discuss the principle, current problems and positive prospects of antifouling strategies in complex sample matrix.

Key words: Electrochemical sensor, Biological sample, Antifouling, Clinical detection

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