Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (8): 963-968.DOI: 10.11944/j.issn.1000-0518.2015.08.140358

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Preparation and Application of the Dry Type Biochemical Film

YANG Huiyu,ZHANG Hao,XU Meiling,GAN Gaolong,CHEN Kele,YU Yuanhua()   

  1. School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
  • Received:2014-10-21 Accepted:2015-05-04 Published:2015-08-08 Online:2015-08-08
  • Contact: YU Yuanhua
  • Supported by:
    Supported by Jilin Provincial Science and Technology Department of Funding Project(No.20140204019SF)

Abstract:

A dry biochemical method for simple and rapid measurement of carbon dioxide-combining capacity, glucose and uric acid in the blood was developed. Appropriate polymeric membrane materials were selected as dry slide carrier. Standard curves were established for the quantization of carbon dioxide-combining capacity, glucose and uric acid by using the dry biochemical analyzer. The dry biochemical analysis method was evaluated by linear test, stability experiment, repetitive experiment and the comparison with wet chemistry method. The three dry slides prepared above have obvious color gradient and color uniformity. The linear range of dry biochemical method established is wide. This method also has a good repeatability as its interassay and intraassay coefficient of variation are all less than 5%. The dry chemistry method and wet chemistry method shows good correlation with the correlation coefficient more than 0.99. The dry slide can be applied to the clinical detection.

 

Key words: dry chemistry, dry slide, clinical detection, dry biochemical method

CLC Number: