Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (12): 1880-1890.DOI: 10.19894/j.issn.1000-0518.220092

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Preparation and Application of Auramine O Imprinted Sensor Based on Nanomaterials Modification

Li QIN1, Xiao-Ting YOU1, Lu-Hua TANG1, Jian-Wen LI2, Yin ZHANG2, Wen-Hui GAO1(), Jun-Hua HAN1()   

  1. 1.College of Food and Biology,Hebei University of Science and Technology,Shijiazhuang 050000,China
    2.Shijiazhuang Food and Drug Inspection Center,Shijiazhuang 050011,China
  • Received:2022-03-26 Accepted:2022-08-06 Published:2022-12-01 Online:2022-12-13
  • Contact: Wen-Hui GAO,Jun-Hua HAN
  • About author:teach2003@126. com
    wenhuigao@126. com
  • Supported by:
    the Key Research and Development Program Project of Hebei Province(19275505D);the Science and Technology Research and Development Plan Project of Shijiazhuang(211170183A)


Auramine O may be illegally used as a food colorant because of its cheapness and easy to enhance color, causing great harm to human health. Therefore, it is of great significance to establish a sensitive and rapid method for the determination of auramine O in food. In the experiment, reduced graphene oxide and gold nanoparticles sol are used to modify working electrodes, a polymer film is formed by cyclic voltammetry on the surface of the modified electrode with auramine O as template molecule, o-aminophenol and o-phenylenediamine as composite functional monomers. The template molecules are eluted using constant potential of -0.1 V in methanol-0.5 mol/L NaOH aqueous solution (1∶1, V/V), the molecularly imprinted electrochemical sensor (MIECS) is prepared successfully for detection of auramine O. Electrochemical analysis method is used to optimize the ratio of template molecules to composite functional monomers. The polymer solution pH and cylinder number, the morphology and chemical structure of the membrane are characterized by scanning electron microscope and infrared spectrometer. The imprinting effect and analysis performance of the sensor are investigated. The results show that the sensor has specific adsorption to auramine O. Under the optimal conditions, the auramine O has a good linear relationship in the concentration range of 1.0×10-9~3.0×10-5 mol/L, the detection limit is 3.3×10-10 mol/L, and the average recoveries are between 91.16% and 101.61% with the relative standard deviations (RSDs) of 2.79%. The sensor has good stability and reproducibility, and is suitable for sensitive and rapid detection of auramine O in food samples.

Key words: Auramine O, Composite functional monomer, Molecularly imprinted electrochemical sensor, Electrochemical analysis, Rapid detection

CLC Number: