叶酸修饰的碳量子点在体外癌细胞成像中的应用

doi:10.11944/j.issn.1000-0518.2020.09.200100

摘要/Abstract

摘要： 具有癌细胞靶向性的荧光纳米探针在生物分析、生物医学和临床诊断等领域有着重要的应用前景。本文基于碳量子点的低毒性、低成本、环境友好、制备方法简单及高发光特性等优点,采用水热法合成了表面富含氨基的荧光碳量子点(CDs),进一步通过1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)缩合的方法,将叶酸(FA)分子中的羧基与其共价连接,从而得到叶酸共价修饰的碳量子点复合材料(FA-CDs)。通过将该复合材料分别与海拉(Hela)和小鼠胚胎成纤维(NIH-3T3)细胞共培养,发现该复合材料能够特异性识别并标记癌细胞,且制备的该复合材料具有低毒性和高发光性等优点。该工作对癌细胞的早期诊断具有重要意义。

关键词: 碳量子点, 叶酸, 纳米生物探针, 靶向, 癌细胞

Abstract: Fluorescent nanobioprobes with cell-targeting specificity are promising to find important applications in bioanalysis, biomedicine, and clinical diagnosis. In this work, folic acid-carbon dot conjugates (FA-CDs) were prepared via hydrothermal method and 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDC) chemistry. The as-prepared FA-CDs with low toxicity and high luminescence can specifically target cancer cells. The specificity of this receptor-mediated internalization was confirmed by comparing the uptake by Hela and NIH-3T3 cells. This work is of great significance for the early diagnosis of cancer cells.

Key words: carbon dots, folic acid, nanobioprobe, targeting, cancer cell

胡国文, 乔玉玲. 叶酸修饰的碳量子点在体外癌细胞成像中的应用[J]. 应用化学, 2020, 37(9): 1003-1009.

HU Guowen, QIAO Yuling. Folic Acid-Carbon Dot Conjugates for Cancer Cell Targeting[J]. Chinese Journal of Applied Chemistry, 2020, 37(9): 1003-1009.

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