石墨烯纳米载药体系的制备及对人口腔鳞癌细胞杀伤效果

doi:10.11944/j.issn.1000-0518.2019.05.180351

摘要/Abstract

摘要：

利用化学氧化还原法制备了氧化石墨烯,进一步超声破碎剥离,得到纳米氧化石墨烯,并对其进行聚乙二醇(PEG)的功能化修饰后载药顺铂。采用扫描电子显微镜(SEM)、紫外-可见吸收光谱(UV-Vis)、傅立叶变换红外光谱(FTIR)对石墨烯纳米载药体系进行表征,细胞存活率实验(MTT)法检验石墨烯纳米载药体系对人口腔鳞癌(KB)细胞的杀伤作用。结果表明,石墨烯纳米载药体系对顺铂的负载率为42.4%,聚乙二醇修饰后可以降低纳米氧化石墨烯的细胞毒性并提高生物相容性,对KB细胞具有双重的杀伤作用,为纳米氧化石墨烯在肿瘤治疗的临床应用提供了理论依据。

关键词: 纳米氧化石墨烯, 顺铂, 细胞存活率实验, 人口腔鳞癌细胞, 细胞毒性

Abstract:

Graphene oxide was synthesized by chemical redox method. The graphene oxide nanoparticles were prepared by ultrasonic fragmentation and peeling, and functionalized by polyethylene glycol(PEG). The modified graphene oxide was loaded with cisplatin. The graphene nano-drug delivery system was characterized by scanning electron microscopy(SEM), ultraviolet-visible absorption spectroscopy(UV-Vis) and Fourier transform infrared spectroscopy(FTIR). The killing effect of graphene nano-drug delivery system on oral squamous cell carcinoma human oral squamous cell carcinoma(KB) cells was examined by cell survival rate experiment(MTT) method. The results demonstrate that the loading rate of graphene nano-drug-loading system is 42.4%. Polyethylene glycol modified nano graphene oxide can reduce the cytotoxicity of nano graphene oxide and improve its biocompatibility. It has dual killing effect on KB cells, which provides a theoretical basis for the clinical application of nano graphene oxide in cancer treatment.

Key words: nano graphene oxide, cisplatin, cell survival rate experiment, human oral squamous cell carcinoma cells, cytotoxicity

张雅静,季鹏,韩德明,赵丽辉,徐亚娟,刘文书. 石墨烯纳米载药体系的制备及对人口腔鳞癌细胞杀伤效果[J]. 应用化学, 2019, 36(5): 564-570.

ZHANG Yajing,JI Peng,HAN Deming,ZHAO Lihui,XU Yajuan,LIU Wenshu. Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells[J]. Chinese Journal of Applied Chemistry, 2019, 36(5): 564-570.

参考文献

[1]	Warnakulasuriya S.Global Epidemiology of Oral and Oropharyngeal Cancer[J]. Oral Oncol,2009,45(4):309-316.
[2]	LUO Yi.Diagnosis and Treatment Strategies of Locally Advanced/Distant Metastasis of Head and Neck Squamous Cell Carcinomas[J]. Anti-tumor Pharm,2016,6(1):1-3(in Chinese). 罗以. 局部晚期/远处转移的头颈部鳞癌的诊治策略[J]. 肿瘤药学,2016,6(1):1-3.
[3]	Yin Q,Chen W.Research Progress of Pathogeny and Treatment of Oral Cancer[J]. Cancer Res Clin,2014,26(8):573-576.
[4]	Nagler R M.Molecular Aspects of Oral Cancer[J]. Anticancer Res,2002,22(5):2977-2980.
[5]	Singh D P,Herrera C E,Singh B,et al. Graphene Oxide:An Efficient Material and Recent Approach for Biotechnological and Biomedical Applications[J]. Mater Sci Eng C,2018,86(3):173-197.
[6]	Banerjee A N.Graphene and Its Derivatives as Biomedical Materials:Future Prospects and Challenges[J]. Interface Focus A Theme Supplement J R Soc Interface,2018,8(3):1-22.
[7]	Bramini M,Alberini G,Colombo E,et al. Interfacing Graphene-Based Materials with Neural Cells[J]. Front Syst Neurosci,2018,12(1):1-22.
[8]	Lee Y,Seo T H,Lee S,et al. Neuronal Differentiation of Human Mesenchymal Stem Cells in Response to the Domain Size of Graphene Substrates[J]. J Biomed Mater Res Part A,2018,106(1):43-51.
[9]	Mohammadrezaei D,Golzar H,Rezai R M,et al. In vitro Effect of Graphene Structures as an Osteoinductive Factor in Bone Tissue Engineering:A Systematic Review[J]. J Biomed Mater Res Part A,2018,106(8):2284-2343.
[10]	Fisher C,Rider A E,Han Z J,et al. Applications and Nanotoxicity of Carbon Nanotubes and Graphene in Biomedicine[J]. J Nanomater,2012,2012(8):1-19.
[11]	JIAO Fang,ZHOU Guoqiang,CHEN Chunying.Trends in Studies on Chemical Modification and Biomedical Applications of Fullerenes[J]. J Ecotoxicol,2010,5(4):469-480(in Chinese). 焦芳,周国强,陈春英. 富勒烯化学修饰与生物医学应用研究进展[J]. 生态毒理学报,2010,5(4):469-480.
[12]	BAI Ru,WANG Wen,JIN Xinglong,et al. Review on Biological Security of Nanomaterials[J]. J Environ Health,2007,24(1):59-61(in Chinese). 白茹,王雯,金星龙,等. 纳米材料生物安全性研究进展[J]. 环境与健康杂志,2007,24(1):59-61.
[13]	Akduman C,Özgüney I,Kumbasar E P A. Preparation and Characterization of Naproxen-Loaded Electrospun Thermoplastic Polyurethane Nanofibers as a Drug Delivery System[J]. Mater Sci Eng C,2016,64(5):383-390.
[14]	Li W D,Sun X Y.Mechanisms by Which Tumor Hypoxic and Acidic Microenvironments Affect Immunotherapy[J]. World Chinese J Digestol,2017,25(21):1934-1944.
[15]	Zhao F,Zhao Y,Liu Y,et al. Cellular Uptake, Intracellular Trafficking, and Cytotoxicity of Nanomaterials[J]. Small,2011,7(10):1322-1337.
[16]	Seabra A B,Paula A J,Lima R D,et al. Nanotoxicity of Graphene and Graphene Oxide[J]. Chem Res Toxicol,2014,27(2):159-168.
[17]	Tian L,Pei X,Zeng Y,et al. Functionalized Nanoscale Graphene Oxide for High Efficient Drug Delivery of Cisplatin[J]. J Nanopart Res,2014,16(11):2709-2722.
[18]	Zhang W,Guo Z,Huang D,et al. Synergistic Effect of Chemo-Photothermal Therapy Using PEGylated Graphene Oxide[J]. Biomaterials,2011,32(33):8555-8561.