二硫化钼纳米材料的制备及其对心肌细胞的保护作用

doi:10.11944/j.issn.1000-0518.2020.09.200042

摘要/Abstract

摘要： 目前,心血管疾病是世界上导致死亡的主要病因,而氧化应激损伤是心血管疾病的重要发病机制之一。本研究旨在探讨二硫化钼(MoS₂)纳米材料在心肌细胞受到氧化应激损伤时的抗氧化保护作用。我们通过一步水热法合成了具备类抗氧化酶活性的MoS₂纳米材料。通过MTT比色法、细胞内凋亡、细胞内活性氧(ROS)检测及Western Blot等实验证实MoS₂纳米材料(100 μg/mL)可以很好地保护H9c2心肌细胞免受双氧水(H₂O₂)诱导的氧化应激损伤。同时,还促进了H9c2心肌细胞的增殖。本研究显示了MoS₂纳米材料可用于构建针对氧化应激损伤引起的心血管疾病的抗氧化剂防御,为纳米药物在抗心肌氧化损伤研发提供实验数据及理论依据。

关键词: 二硫化钼纳米材料, 类抗氧化酶活性, 氧化应激, 活性氧, 心肌保护

Abstract: Cardiovascular diseases currently dominate the cause of death worldwide. Oxidative stress injury is one of the important pathogenesis of cardiovascular diseases. The purpose of this study is to investigate the antioxidative protection effect of molybdenum disulfide (MoS₂) nanoparticles on cardiomyocytes under oxidative stress injury. We synthesized MoS₂ nanoparticles with antioxidant-like enzyme activity by a one-step hydrothermal method. MTT, intracellular apoptosis, intracellular reactive oxygen species (ROS) detection, and Western Blot experiments confirm that MoS₂ nanoparticles (100 μg/mL) can protect H9c2 cardiomyocytes from oxidative stress injury induced by hydrogen peroxide (H₂O₂). At the same time, it also promotes the proliferation of H9c2 cardiomyocytes. This study shows that MoS₂ nanoparticles can be used to construct antioxidant defense against cardiovascular diseases caused by oxidative stress injury, and provide experimental data and theoretical basis for the development of nanomedicine in the resistance to myocardial oxidative damage.

Key words: molybdenum disulfide nanoparticles, antioxidant enzyme-like activity, oxidative stress, reactive oxygen species, myocardial protection

高明, 赵开东, 刘祥永, 金元哲. 二硫化钼纳米材料的制备及其对心肌细胞的保护作用[J]. 应用化学, 2020, 37(9): 1010-1021.

GAO Ming, ZHAO Kaidong, LIU Xiangyong, JIN Yuanzhe. Preparation of Molybdenum Disulfide Nanoparticles and the Cytoprotection on Cardiac Myocytes[J]. Chinese Journal of Applied Chemistry, 2020, 37(9): 1010-1021.

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