应用化学 ›› 2019, Vol. 36 ›› Issue (2): 212-222.DOI: 10.11944/j.issn.1000-0518.2019.02.180161

• 研究论文 • 上一篇    下一篇

邻菲啰啉并1,2,4-三嗪钴(Ⅲ)配合物的合成及其对小牛胸腺DNA的荧光识别

张成路(),王静,李静怡,于向坤,杨敬怡,柴金华,李益政,王华玉,宫荣庆   

  1. 辽宁师范大学化学化工学院 辽宁 大连 116029
  • 收稿日期:2018-05-08 出版日期:2019-02-01 发布日期:2019-01-31
  • 通讯作者: 张成路
  • 基金资助:
    辽宁省教育厅科学技术项目资助(2009A426)

Synthesis of 1,2,4-Triazine-PhenanthrolineCo(Ⅲ) Complexes and Their FluorescenceRecognition on Calf Thymus DNA

ZHANG Chenglu(),WANG Jing,LI Jingyi,YU Xiangkun,YANG Jingyi,CAI Jinhua,LI Yizhen,WANG Huayu,GONG Rongqing   

  1. College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian,Liaoning 116029,China
  • Received:2018-05-08 Published:2019-02-01 Online:2019-01-31
  • Contact: ZHANG Chenglu
  • Supported by:
    Supported by Technology Research Program of Liaoning Provincial Department of Education(No.2009A426)

摘要:

在4个邻菲啰啉并1,2,4-三嗪新型配体分子(ARTP1ARTP4)合成的基础上,通过ARTP1ARTP4与Co3+配位,制备了4个钴(Ⅲ)配合物(Co-ARTP1Co-ARTP4)。 通过傅里叶变换红外光谱仪(FT-IR)、核磁共振波谱仪(NMR)和高分辨质谱(HRMS)仪和紫外-可见分光计(UV-Vis)等技术手段表征了ARTP1ARTP4和Co-ARTP1Co-ARTP4的结构和性能。 结果表明,配合物的紫外吸收峰中出现减色效应,最大吸收峰红移。 配合物以插入的方式与小牛胸腺DNA结合,结合常数Kb分别为4.78×105、6.52×105、5.97×105和6.01×105 L/mol,表明配合物可作为潜在的CT-DNA荧光探针,为探索DNA与有机小分子的作用提供了重要的参考。

关键词: 邻菲啰啉, 1,2,4-三嗪, 小牛胸腺DNA, 荧光探针, 钴配合物

Abstract:

Four 1,2,4-triazine-phenanthroling derivatives(ARTP1ARTP4) were designed and synthesized successfully. ARTP1ARTP4 were selected to coordinate with Co3+ to afford four novel complexes(Co-ARTP1Co-ARTP4). The structures and properties of ARTP1ARTP4 and Co-ARTP1Co-ARTP4 were studied by means of Fourier transform infrared spectrometer(FT-IR), nuclear magnetic resonance(NMR), high resolution mass spectrometry(HRMS) and ultraviolet-visible spectroscopy(UV-Vis). As a result, the excitation peaks of the complexes are weakened and show red shift. The complexes interact with calf thymus DNA(CT-DNA) through the insert mode and the binding constants are Kb=4.78×105, 6.52×105, 5.97×105 and 6.01×105 L/mol, respectively, which indicates that the complexes are expected to be the DNA fluorescence probes. It provides the important theory references for further study on the interaction between small organic molecules with DNA.

Key words: phenanthroline, 1,2,4-triazine, calf thymus DNA, fluorescence probe, Co-complexes