Heparin/Barium Titanate-Loaded PVDF Electrospun Membranes： Fabrication， Anticoagulant， and Antibacterial Performance

doi:10.19894/j.issn.1000-0518.250091

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (12): 1636-1648.DOI: 10.19894/j.issn.1000-0518.250091

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Heparin/Barium Titanate-Loaded PVDF Electrospun Membranes： Fabrication， Anticoagulant， and Antibacterial Performance

Chuan-Yu GUO¹^,², Li-Yu SUN¹^,², Xing-Hua GUAN¹^,²(), Qiang SHI¹^,²()

^1.School of Applied Chemistry and Engineering，University of Science and Technology of China，Hefei 230026，China
^2.State Key Laboratory of Polymer Science and Technology，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022，China

Received:2025-03-04 Accepted:2025-08-13 Published:2025-12-01 Online:2025-12-30
Contact: Xing-Hua GUAN,Qiang SHI

Abstract

Abstract:

Blood-contacting medical devices hold significant clinical applications， including vascular stents， artificial blood vessels， valves， and blood purification equipment， playing a vital role in safeguarding human health. However， these devices often trigger immune responses and thrombus formation during use， leading to suboptimal therapeutic outcomes. Additionally， bacterial contact on device surfaces during implantation may result in infections. Consequently， there is an urgent need to develop blood-contacting device surfaces with dual anticoagulant and antibacterial functionalities. This study addresses the issues of thrombosis and bacterial infections that can occur during the use of blood contact devices. Using electrospinning technology， a polyvinylidene fluoride （PVDF） micro-nano fiber membrane loaded with heparin and barium titanate （PVDF/Hep/BaTiO₃） was prepared on the surface of a substrate material. The material's surface was characterized using scanning electron microscopy， Fourier transform infrared spectroscopy， and X-ray photoelectron spectroscopy. The study investigated the biocompatibility， hemocompatibility， and antibacterial properties of the constructed surface. The results showed that the micro-nano fibers containing heparin exhibit excellent anti-thrombotic properties， reducing platelet adhesion by 60%. The extracellular matrix-like structure of the micro-nano fibers， combined with heparin， enhances the material's ability to promote endothelial cell proliferation by 20%. Additionally， the hemolysis rate of the micro-nano fibers is less than 5%， and the cell viability is over 90%， indicating good biocompatibility. Furthermore， the barium titanate and PVDF in the micro-nano fibers work together to enhance the piezoelectric effect， killing over 90% of bacteria after ultrasonic pretreatment.Thus， the PVDF/Hep/BaTiO₃ micro/nano fiber-modified membrane exhibits excellent cell proliferation promotion， anticoagulation， and antibacterial properties， providing a simple yet effective surface modification strategy for multifunctional optimization of blood-contacting medical devices.

Key words: Heparin, Polyvinylidene fluoride, Anticoagulant, Antibacterial, Piezoelectric effect

CLC Number:

O631

Chuan-Yu GUO, Li-Yu SUN, Xing-Hua GUAN, Qiang SHI. Heparin/Barium Titanate-Loaded PVDF Electrospun Membranes： Fabrication， Anticoagulant， and Antibacterial Performance[J]. Chinese Journal of Applied Chemistry, 2025, 42(12): 1636-1648.

Figures/Tables 13

Fig.1 SEM image of the surface of （A） PU，（B） PVDF and （C） PVDF/Hep/BaTiO3； Diameter size and distribution of （D） PVDF and （E） PVDF/Hep/BaTiO3

Fig.2 FT-IR spectra of PU， PVDF， PVDF/Hep/BaTiO3 and BaTiO3

Fig.3 XPS and F1s spectra of PVDF and PVDF/Hep/BaTiO3

Fig.4 Water contact angle analysis of PU， PVDF and PVDF/Hep/BaTiO3

Fig.5 （A） Piezoelectric voltage and （B） piezoelectric current of PVDF and PVDF/Hep/BaTiO3

Fig.6 Live/dead fluorescence staining images of L929 cells co-cultured with PU， PVDF and PVDF/Hep/BaTiO3 for 24 h （green fluorescence represents Calcein AM， which is live cells； red fluorescence represents PI， which is dead cells）

Fig.7 Viability of L929 cells was determined after co-cultivation with PU， PVDF and PVDF/Hep/BaTiO3 for 24 and 48 h

Fig.8 Hemolysis analysis of PU， PVDF and PVDF/Hep/BaTiO3

Fig.9 Morphology of HUVEC on the surface of samples after co-cultivation with PU， PVDF and PVDF/Hep/BaTiO3 for 24 and 48 h was observed under laser confocal microscope

Fig.10 Proliferation of HUVEC cells after co-cultivation with PU， PVDF and PVDF/Hep/BaTiO3 for 24 and 48 h

Fig.11 （A） Protein adhesion data on the surfaces and （B） blood clotting index of PU， PVDF and PVDF/Hep/BaTiO3

Fig.12 Half quantitative results of platelet adhesion of PU， PVDF and PVDF/Hep/BaTiO3（490 nm）

Fig.13 Pictures and efficiency of antibacterial effect for PU， PVDF and PVDF/Hep/BaTiO3 samples against S.aureus and E.coli before and after ultrasonic treatment

References 29

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Heparin/Barium Titanate-Loaded PVDF Electrospun Membranes： Fabrication， Anticoagulant， and Antibacterial Performance

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