Advances in Construction and Electrochemical Biosensing of Antifouling Interfaces

doi:10.19894/j.issn.1000-0518.210144

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (5): 736-748.DOI: 10.19894/j.issn.1000-0518.210144

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Advances in Construction and Electrochemical Biosensing of Antifouling Interfaces

Zhen-Hua LI¹^,³, Ying ZHU¹^,², Jing CHEN¹^,², Shi-Ping SONG¹^,²()

^1.Division of Physical Biology，CAS Key Laboratory of Interfacial Physics and Technology，Shanghai Institute of Applied Physics，Chinese Academy of Sciences，Shanghai 201800，China
^2.The Interdisciplinary Research Center，Shanghai Synchrotron Radiation Facility，Zhangjiang Laboratory，Shanghai Advanced Research Institute，Chinese Academy of Sciences，Shanghai 201210，China
^3.University of Chinese Academy of Sciences，Beijing 100049，China

Received:2021-03-25 Accepted:2021-06-23 Published:2022-05-01 Online:2022-05-24
Contact: Shi-Ping SONG
About author:songsp@sari.ac.cn
Supported by:
the National Key R&D Program of China(2018YFF0212803);the National Natural Science Foundation of China(21974147)

Abstract

Abstract:

Electrochemical biosensors have made rapid development in the field of wearable healthcare monitoring and have great potential applications in clinical detection due to high sensitivity， good portability， fast response and easy integration. However， non-specific adsorption of non-target biological substances on the electrode surface （i.e.， biofouling） when exposed to the actual clinical biological sample affects the performance of electrochemical biosensors. Therefore， the construction of a sensing interface with antifouling capability （antifouling interface） to prevent non-target substances from adsorbing to the electrode surface is of great importance for expanding the practical application range of electrochemical sensors and realizing detection in complex biological samples. In this review， antifouling electrode interfaces based on physical， chemical， or biological strategies and their application in clinical biomarker detection are summarized， which provides technical references for the improvement of electrochemical biosensors in practical application. Finally， we discuss the principle， current problems and positive prospects of antifouling strategies in complex sample matrix.

Key words: Electrochemical sensor, Biological sample, Antifouling, Clinical detection

CLC Number:

O652

Zhen-Hua LI, Ying ZHU, Jing CHEN, Shi-Ping SONG. Advances in Construction and Electrochemical Biosensing of Antifouling Interfaces[J]. Chinese Journal of Applied Chemistry, 2022, 39(5): 736-748.

Figures/Tables 6

References 74

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抗污策略	抗污原理	抗污组成	靶标分子	检测基质	线性范围	检测限	参考文献
Antifouling strategies	Principle	Antifouling component	Target	Matrix	Linear range	Detection limit	Ref.
物理抗污 Physical	孔径限制 Pore size restriction and filtration	纳米多孔金	脱氧核糖核酸	牛血清白蛋白	10~200 nmol/L	－ ^a	［15］
		Nanoporous gold film	DNA	BSA	10~200 nmol/L	－ ^a	［15］
		纳米多孔氧化铝膜	癌抗原15?3	全血	60~240 U/mL	52 U/mL	［20］
		AAO nanoporous membrane	CA15?3	Blood	60~240 U/mL	52 U/mL	［20］
化学抗污Chemical	水化层 Hydration layer	PEG和糖胺聚糖抗污层	人乳头瘤病毒DNA	100%血清	0.001~10 nmol/L	－	［35］
		PEG and CSA layer	HPV DNA	100% serum	0.001~10 nmol/L	－	［35］
		苯基磷酰胆碱抗污层	肿瘤坏死因子α	磷酸缓冲盐溶液	0.1~150 pg/mL	0.1 pg/mL	［46］
		PPC layer	TNF?α	PBS	0.1~150 pg/mL	0.1 pg/mL	［46］
生物抗污Biological	空间位阻	DNA纳米结构	凝血酶	磷酸缓冲盐溶液	100~ 100000 pmol/L	100 pmol/L	［59］
	Steric hindrance	DNA nanostructure	Thrombin	PBS	100~ 100000 pmol/L	100 pmol/L	［59］
	水化层	多功能肽	免疫球蛋白G	血清	－	0.26 ng/mL	［69］
	Hydration layer	Multifunctional peptide	Immunoglobulin G	Serum	－	0.26 ng/mL	［69］
	孔径限制	多孔纳米复合物涂层	白细胞介素6	血浆	－	23 pg/mL	［70］
	Pore size restriction	Porous nanocomposite coating	Interleukin 6	Plasma	－	23 pg/mL	［70］

抗污策略	抗污原理	抗污组成	靶标分子	检测基质	线性范围	检测限	参考文献
Antifouling strategies	Principle	Antifouling component	Target	Matrix	Linear range	Detection limit	Ref.
物理抗污 Physical	孔径限制 Pore size restriction and filtration	纳米多孔金	脱氧核糖核酸	牛血清白蛋白	10~200 nmol/L	－ ^a	［15］
		Nanoporous gold film	DNA	BSA	10~200 nmol/L	－ ^a	［15］
		纳米多孔氧化铝膜	癌抗原15?3	全血	60~240 U/mL	52 U/mL	［20］
		AAO nanoporous membrane	CA15?3	Blood	60~240 U/mL	52 U/mL	［20］
化学抗污Chemical	水化层 Hydration layer	PEG和糖胺聚糖抗污层	人乳头瘤病毒DNA	100%血清	0.001~10 nmol/L	－	［35］
		PEG and CSA layer	HPV DNA	100% serum	0.001~10 nmol/L	－	［35］
		苯基磷酰胆碱抗污层	肿瘤坏死因子α	磷酸缓冲盐溶液	0.1~150 pg/mL	0.1 pg/mL	［46］
		PPC layer	TNF?α	PBS	0.1~150 pg/mL	0.1 pg/mL	［46］
生物抗污Biological	空间位阻	DNA纳米结构	凝血酶	磷酸缓冲盐溶液	100~ 100000 pmol/L	100 pmol/L	［59］
	Steric hindrance	DNA nanostructure	Thrombin	PBS	100~ 100000 pmol/L	100 pmol/L	［59］
	水化层	多功能肽	免疫球蛋白G	血清	－	0.26 ng/mL	［69］
	Hydration layer	Multifunctional peptide	Immunoglobulin G	Serum	－	0.26 ng/mL	［69］
	孔径限制	多孔纳米复合物涂层	白细胞介素6	血浆	－	23 pg/mL	［70］
	Pore size restriction	Porous nanocomposite coating	Interleukin 6	Plasma	－	23 pg/mL	［70］

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Advances in Construction and Electrochemical Biosensing of Antifouling Interfaces

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