Facile One⁃Step Synthesis of WS2@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application

doi:10.19894/j.issn.1000-0518.210180

Chinese Journal of Applied Chemistry ›› 2022, Vol. 39 ›› Issue (3): 480-488.DOI: 10.19894/j.issn.1000-0518.210180

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Facile One⁃Step Synthesis of WS₂@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application

Hong-Zhen PENG¹^,³^,⁴, Yu ZHANG¹^,³, Lin-Jie GUO¹^,³, Wei SONG²(), Qing-Nuan LI¹(), Xiang-Ying MENG²()

^1.Division of Physical Biology，CAS Key Laboratory of Interfacial Physics and Technology，Shanghai Institute of Applied Physics，Chinese Academy of Sciences，Shanghai 201800，China
^2.School of Medical Laboratory，Weifang Medical University，Weifang 261053，China
^3.The Interdisciplinary Research Center，Shanghai Synchrotron Radiation Facility，Zhangjiang Laboratory，Shanghai Advanced Research Institute，Chinese Academy of Sciences，Shanghai 201210，China
^4.University of Chinese Academy of Sciences，Beijing 100049，China

Received:2021-04-10 Accepted:2021-07-15 Published:2022-03-01 Online:2022-03-15
Contact: Wei SONG,Qing-Nuan LI,Xiang-Ying MENG
About author:wysw8103@163.com； liqingnuan@sinap.ac.cn； mengxiangying85730@126.com
Supported by:
the Medical and Health Science and Technology Development Program of Shandong Province(202012060616);the Natural Science Foundation of Shandong Province(ZR2016BL19)

1. Facile One-Step Synthesis of WS2@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application.pdf(257KB)

Abstract

Abstract:

The interface modification of electrochemical sensors is an important way to improve their detection performance. Among them， enhancing the biocompatibility and electrical conductivity of the electrochemical sensing interface is a major challenge for the development of electrochemical sensors. In this work， the surface of glassy carbon electrode is functionalized based on WS₂@Au quantum dots （WS₂@Au QDs） prepared by in situ one-step reduction， which is used to immobilize oxidoreductase， thus realizing the construction of high-performance biosensors. By virtue of the excellent biocompatibility and electrical conductivity of WS₂@Au QDs， the WS₂@Au QDs can effectively protect the catalytic activity of GOx and promote the direct electron transfer between GOx and the electrode， and the electron transfer rate constant reaches 2.25 s^-1. Based on the good electrocatalytic effect of the sensor on glucose， the method has been successfully applied to the detection of glucose， with a linear range of 5~50 μmol/L and a detection limit of 1.5 μmol/L， and the sensor shows good selectivity， reproducibility and stability. These results suggest that the WS₂@Au QDs has potential important application prospects in the interface modification of biosensors， which lays a good research foundation for the construction of high-performance biosensors.

Key words: WS₂@Au quantum dots, In situ reduction, Direct electrochemistry, Glucose oxidase, Biosensor

CLC Number:

O646

Hong-Zhen PENG, Yu ZHANG, Lin-Jie GUO, Wei SONG, Qing-Nuan LI, Xiang-Ying MENG. Facile One⁃Step Synthesis of WS₂@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application[J]. Chinese Journal of Applied Chemistry, 2022, 39(3): 480-488.

Figures/Tables 5

Fig.1 （A） Schematic illustration for preparation of WS2@Au QDs. TEM images （B and C） and EDS （D and E） of WS2 QDs and WS2@Au QDs， respectively

Fig.2 （A） UV-Vis absorption spectra of WS2 QDs （a） and WS2@Au QDs （b）. Inset： the magnified UV-Vis absorption peak of Au NPs. （B） UV-Vis absorption spectra of GOx （a） and GOx/WS2@Au QDs （b）

Fig.3 （A） CVs of bare GCE （a）， WS2@Au QDs-N afion/GCE （b）， GOx-Nafion/GCE （c）， GOx/WS2 QDs-Nafion/GCE （d）， and GOx/WS2@Au QDs-Nafion/GCE （e） in 0.1 mol/L pH=7.0 N2-saturated PBS at a scan rate of 100 mV/s. （B） Schematic illustration of the DET of GOx on the modified GCE. （C） The effect of scan rate on the direct electron transfer of GOx/WS2@Au QDs-Nafion/GCE. Plots of the anodic and cathodic peak currents vs. the scan rate. （D） The effect of electrolyte pH on the DET of GOx/WS2@Au QDs-Nafion/GCE. The plot of formal potential with pH

Fig.4 Electrocatalytic performance of the WS2@Au QD-based electrochemical biosensor（A） Cyclic voltammograms of GOx/WS2@Au QDs-Nafion/GCE in nitrogen-saturated （a）， air-saturated （b）， and oxygen-saturated PBS buffer （0.1 mol/L，pH=7.0） in the absence （c） and presence （d） of glucose， respectively. （B） Dependence of the decreased reduction peak current of the GOx/WS2@Au QDs-Nafion/GCE on the concentration of glucose. （C） Selectivity of our assay system for the determination of glucose

Table 1 Comparison of the analytical performances of different glucose biosensors

检测电极 Detection electrode	线性范围 Linear range/（μmol·L^-1）	检测限 LOD/（μmol·L^-1）	参考文献 Reference
GOx/TiO₂ NSA/carbon cloth electrode	100~500	23.4	［46］
GOx?Ag@C/Nafion/GCE	50~2500	20	［33］
Graphene/AuNPs/GOx/chitosan/gold electrode	2 000~14 000	180	［47］
GOx?PDA?Au?Fe₃O₄/MGCE	20~1875	6.5	［35］
GOx/NCNSs@CNFs/GCE	12~1 000	2	［48］
GOx/cage?like?PbS/Nafion/GCE	50~1 450	10	［49］
GOx?X?APTES/BDD	15~400	10	［9］
GOx/WS₂@Au QDs?Nafion/GCE	5~400	1.5	本工作 This work

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Facile One⁃Step Synthesis of WS₂@Au Quantum Dot Composite by in situ Reduction and Its Sensing Application

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