One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition

doi:10.11944/j.issn.1000-0518.2015.07.140401

Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (7): 849-854.DOI: 10.11944/j.issn.1000-0518.2015.07.140401

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One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition

YANG Shaoming^*, CHEN Yansheng, LI Ruiqin, LONG Qiyang, DING Suyou

Department of Chemistry and Chemical Engineering,School of Sciences, East China Jiaotong University,Nanchang 330013,China

Received:2014-11-19 Accepted:2015-02-14 Published:2015-06-30 Online:2015-06-30
Contact: Shaoming YANG
Supported by:
Supported by the National Natural Science Foundation of China (No.21465012, No.21065004)

Abstract

Abstract:

Horseradish peroxidase (HRP) and carbon nanotubes (CNTs) were co-immobilized on the base electrode surface during the process of potentiostatic electrodeposition of cobalt-aluminium layered double hydroxide (CoAl-LDH). The as-prepared CoAl-LDH-HRP-CNTs modified electrode was used for the determination of hydrogen peroxide. The morphology of CoAl-LDH-HRP-CNTs was characterized by SEM. The preparation process of the modified electrode was monitored by the electrochemical spectroscopy. The electrochemical behavior of the modified electrode was studied by cyclic voltammetry. The effect of pH and work potential on the catalytic reduction performance of the modified electrode was investigated. The biosensor shows a good linear range of 2.5×10^-6~3.35×10^-4 mol/L for the determination of hydrogen peroxide with the sensitivity of 0.0049 A·L/mol.

Key words: cobalt-aluminium layered double hydroxide, carbon nanotubes, horseradish peroxidase, electrodeposition, biosensor

CLC Number:

YANG Shaoming, CHEN Yansheng, LI Ruiqin, LONG Qiyang, DING Suyou. One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition[J]. Chinese Journal of Applied Chemistry, 2015, 32(7): 849-854.

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One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition

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