Synthesis and Drug Loading Performance of pH-Responsive Magnetic Nanocomposites

doi:10.19894/j.issn.1000-0518.230359

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (6): 861-869.DOI: 10.19894/j.issn.1000-0518.230359

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Synthesis and Drug Loading Performance of pH-Responsive Magnetic Nanocomposites

Ling-Xiao WANG¹^,², Shan GAO², Wan-Yu WEI², Ning-Bo LI²(), Jie QIAO²(), Chuan DONG³

^1.Department of Chemistry and Chemical，Taiyuan Institute of Technology，Taiyuan 030008，China
^2.School of Basic Medical Sciences，Shanxi Medical University，Taiyuan 030001，China
^3.Institute of Environmental Science，Shanxi University，Taiyuan 030006，China

Received:2023-11-14 Accepted:2024-03-12 Published:2024-06-01 Online:2024-07-09
Contact: Ning-Bo LI,Jie QIAO
About author:qiaojie@sxmu.edu.cn
ningboli@sxmu.edu.cn；
Supported by:
the Youth Foundation of Taiyuan Institute of Technology(2020LG02);Shanxi Scholarship Council of China(2021-077);the Natural Science Foundation of Shanxi Province of China(202203021211240)

Abstract

Abstract:

Mesoporous silica-coated magnetic nanoparticles were prepared using a modified St?ber method， followed by amino-functionalization treatment on the material surface through grafting to form NH₂-MMNPs. A safe and non-toxic oxidized pullulan was used as the gated material， forming a pH-responsive imine bond between the amino groups on the surface of the nanomaterial and the aldehyde groups of oxidized pullulan， thus creating a gated switch. The loaded doxorubicin hydrochloride was encapsulated to form the intelligent drug delivery system （oMMNPs/DOX）. The structure， properties， and morphology of the composite material were characterized by transmission electron microscopy （TEM）， X-ray diffraction spectroscopy （XRD）， fourier transform infrared spectroscopy （FT-IR）， nitrogen adsorption desorption （BET） and vibrating sample magnetometry （VSM）. The results showed that the composite material had good morphology， monodispersity， and superparamagnetism. Drug loading and release experiments indicated a drug loading capacity of 51.65% for NH₂-MMNPs； oMMNPs/DOX exhibited pH-responsive drug release behavior and sustained drug release during the release process. Cytotoxicity and fluorescence imaging experiments demonstrated that the composite material had good biocompatibility， successfully loaded drugs， and were uptaken by cells.

Key words: Pullulan oxide, Magnetic nanoparticles, Mesoporous silica, pH response, Doxorubicin

CLC Number:

O651

Ling-Xiao WANG, Shan GAO, Wan-Yu WEI, Ning-Bo LI, Jie QIAO, Chuan DONG. Synthesis and Drug Loading Performance of pH-Responsive Magnetic Nanocomposites[J]. Chinese Journal of Applied Chemistry, 2024, 41(6): 861-869.

Figures/Tables 8

Fig.1 Synthesis route and drug release schematic diagram of pH-responsive magnetic nanocomposite

Fig.2 （A） TEM images of the NH2-MMNPs；（B， C） TEM images of the oMMNPs/DOX；（D） Size distributions of MNPs and NH2-MMNPs

Fig.3 （A） FT-IR spectra of PL and oxPL；（B） FT-IR spectra of MNPs， NH2-MMNPs， oMMNPs and oxPL

Fig.4 Nitrogen adsorption-desorption isotherm（A） and pore size distribution （B） curves of NH2-MMNPs； X-ray diffraction patterns （C） and magnetization curves （D） of MNPs， NH2-MMNPs and oMMNPs/DOX

Fig.5 （A） Standard curve of DOX；（B） Isothermal adsorption curve of NH2-MMNPs for DOX （25 ℃）

Fig.6 Release curves of oMMNPs/DOX in different pH conditions

Fig.7 Cell viabilities of HepG2 and HCT116 cells incubated with varied concentrations of oMMNPs （A）， oMMNPs/DOX （B） and free DOX （C）

Fig.8 Fluorescent inverted microscope images of HCT116 cells treated with oMMNPs/DOX （scale bar： 100 μm）

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Synthesis and Drug Loading Performance of pH-Responsive Magnetic Nanocomposites

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