Preparation of CO2 Stimulus-Responsive Hydrogel and Its Application in Protein Separation

doi:10.19894/j.issn.1000-0518.230255

Chinese Journal of Applied Chemistry ›› 2024, Vol. 41 ›› Issue (6): 870-877.DOI: 10.19894/j.issn.1000-0518.230255

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Preparation of CO₂ Stimulus-Responsive Hydrogel and Its Application in Protein Separation

Shou-Cun ZHANG(), Xue-Yin YAN, Yang-Hua SONG, Zhuo-Min LI

Key Laboratory of Polymer Materials and Manufacturing Technology，College of Materials Science and Engineering，North Minzu University，Yinchuan 750021

Received:2023-08-26 Accepted:2024-05-06 Published:2024-06-01 Online:2024-07-09
Contact: Shou-Cun ZHANG
About author:zhang_sc@ nun.edu.cn
Supported by:
Key Laboratory of Powder Material & Advanced Ceramics North Minzu University(2201);Ningxia Natural Science Foundation(2023AAC03282)

Abstract

Abstract:

A composite hydrogel material， denoted as GCel/PD， is synthesized by utilizing cellulose， N，N′-dimethylaminoethyl methacrylate （DMAEMA） and N，N'-methylenebis（acrylamide） as the main raw materials. The results demonstrate that the GCel/PD materials exhibit significant CO₂ stimulus-responsive characteristics， and can effectively adsorb and separate bovine serum albumin （BSA）. The maximum adsorption capacity can reach （434.4±15） mg/g. Moreover， under the cyclic switching between CO₂ and nitrogen atmospheres， the GCel/PD material can exhibit good cycling stability. The adsorption capacity is as high as 167.5 mg/g even after three usage cycles. The material's particulate nature facilitates its post-usage separation from the system， exhibits promising potential in protein extraction and separation.

Key words: Composite hydrogel, CO₂, Stimulus-responsive, Adsorption and separation

CLC Number:

O636

Shou-Cun ZHANG, Xue-Yin YAN, Yang-Hua SONG, Zhuo-Min LI. Preparation of CO₂ Stimulus-Responsive Hydrogel and Its Application in Protein Separation[J]. Chinese Journal of Applied Chemistry, 2024, 41(6): 870-877.

Figures/Tables 8

Table 1 Preparation recipes of GCel/PD composite hydrogels

Sample	m（GCel）/g ^a	c（DMAEMA）/（mol·L^-1） ^b	c（MBA）/（mol·L^-1） ^b	c（APPH）/（mol·L^-1） ^b	V（H₂O）/mL
GCel/PD-1	5.0	2.32	0.173	0.153	4.0
GCel/PD-2	5.0	1.89	0.141	0.125	4.0
GCel/PD-3	5.0	1.39	0.104	0.092	4.0
GCel/PD-4	5.0	0.771	0.058	0.051	4.0

Fig.1 Preparation process of cellulose-based composite hydrogel materials and the adsorption/desorption process of BSA by the switch of CO2/N2

Fig.2 FT-IR spectra of PDMAEMA， GCel and GCel/PD

Fig.3 SEM images of （A） GCel，（B） GCel/PD-1，（C） GCel/PD-2，（D） GCel/PD-3，（E） GCel/PD-4 and （F） GCel/PD-2 after three adsorption/desorption cycles

Table 2 Results of BET test and Zeta potential analysisof samples， as well as the pH of the GCel/PD-BSA suspension solution saturated with CO2

Samples	Pore diameter/nm	Specific surface area/（m²·g^-1）	Zeta potential/mV ^a	pH
GCel	4.6	18.59	2.07/1.74	4.42
GCel/PD-1	5.6	16.64	6.63/-1.85	5.21
GCel/PD-2	4.5	22.70	6.48/-6.99	5.54
GCel/PD-3	4.3	21.72	-4.51/-10.67	5.29
GCel/PD-4	5.2	24.45	-5.45/-18.87	5.13

Fig.4 Pore size distribution （BJH） of GCel and GCel/PD composite hydrogel materials

Fig.5 Adsorption of BSA by GCel and GCel/PD composite materials

Fig.6 BSA cyclic adsorption capacity under CO2 and N2 cycles； Solution concentration variation

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Preparation of CO₂ Stimulus-Responsive Hydrogel and Its Application in Protein Separation

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