Abstract:

Hypoxanthine, xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector. This process of sample preparation was improved and the conditions of HPLC were also optimized. The detection limits of hypoxanthine, xanthine and adenine were 0.012×10-6, 0.041×10-6, 0.042×10-6 g/mL, respectively. The intra-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 4.1%, 7.3% and 4.5%, respectively. The inter-day precisions(RSD) of hypoxanthine, xanthine and adenine were less than 5.8%, 2.9% and 3.1%, respectively. The recovery rates of the four samples with added specimen were in a range of 94% to 107%.

Key words: high performance liquid chromatography,ultrasound extraction，purine,animal drugs