Chinese Journal of Applied Chemistry

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Application of Rapid Biochemical Oxygen Demand Biosensor

ZHAO Limin1, JIA Jianbo2, LIU Changyu2*   

  1. (1.Changchun Environmental Monitoring Center,Changchun 130022,China;
    2.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)
  • Received:2011-11-09 Revised:2012-01-16 Published:2012-07-10 Online:2012-07-10

Abstract: Multiple-species BODseed was used for fabricating biochemical oxygen demand(BOD) biosensors for rapid online BOD determination. Some factors to accuracy were studied such as calibration solution selection, sample self-biodegradation, and thermostatic device. Good results were obtained when wastewater was used as calibration solution instead of glucose and glutimic acid(GGA). The self-biodegradation process made the rapid measurements inaccurate. Besides, thermostatic device used only for biosensors is defective, and it should be applied for maintaining a constant temperature of the samples and buffer, promoting the efficiency and accuracy of online monitoring.

Key words: Biochemical oxygen demand, Biosensor, Thermostatic device, Online, Calibration solution

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