ELISA detection of Steroidal Hormones in Water Resources, Foods and Feed

doi:10.3724/SP.J.1095.2010.00067

Chinese Journal of Applied Chemistry ›› 2010, Vol. 27 ›› Issue (12): 1466-1469.DOI: 10.3724/SP.J.1095.2010.00067

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ELISA detection of Steroidal Hormones in Water Resources, Foods and Feed

MA De-Zhang¹, DIAO Yu-Wen², YANG Jia-Xin¹, WANG Bao-De³, YU Yuan-Hua^1*

(1.School of Life Science and Technology,Changchun University of Science and Technology，Changchun 130022;
2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun;
3.Changchun Corporation of Chinese Oil Transportation Pipeline,Changchun)

Received:2010-01-26 Revised:2010-05-14 Published:2010-12-10 Online:2010-12-10

Abstract

Abstract:

Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones, then it has the characteristics to degrade steroid hormones. This work developed a Enzyme-linked immunosorbent assay(ELISA) method to detect the steroidal hormones in water, food and animal feed. The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship. The linear range is within 0.5~600 μg/L. Adopting this method, the study detect the steroidal hormones in water, food and feed. Content of testosterone in Songhua Lake is 34.5 μg/L, which is higher than others. Content of testosterone in the 8 animal feed is more than 2 μg/L. Meat sample test shows that the amount of steroidal hormones is below the detection limit. This result from this method is consistent with that from HPLC.

Key words: steroid hormones, ELISA, 3α-HSD/CR

CLC Number:

O652

MA De-Zhang1, DIAO Yu-Wen2, YANG Jia-Xin1, WANG Bao-De3, YU Yuan-Hua1*. ELISA detection of Steroidal Hormones in Water Resources, Foods and Feed[J]. Chinese Journal of Applied Chemistry, 2010, 27(12): 1466-1469.

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ELISA detection of Steroidal Hormones in Water Resources, Foods and Feed

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