Abstract:

Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones, then it has the characteristics to degrade steroid hormones. This work developed a Enzyme-linked immunosorbent assay(ELISA) method to detect the steroidal hormones in water, food and animal feed. The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship. The linear range is within 0.5~600 μg/L. Adopting this method, the study detect the steroidal hormones in water, food and feed. Content of testosterone in Songhua Lake is 34.5 μg/L, which is higher than others. Content of testosterone in the 8 animal feed is more than 2 μg/L. Meat sample test shows that the amount of steroidal hormones is below the detection limit. This result from this method is consistent with that from HPLC.

Key words: steroid hormones, ELISA, 3α-HSD/CR