Abstract:

In a pH=6.9 BR buffer medium, brilliant cresyl blue reacted with inosine to form ionassociation complex.The maximum fading wavelength was located at 630 nm. The fading intensity is proportional to the concentration of inosine. The concentration of inosine in a range of 0.18×10-6~6.0×10-6 g/mL obeys Beer′s Law. The correlation coefficient is 0.999 3, the molar absorptivity is 1.13×104 L/(mol·cm), the detection limit is 53.5×10-9 g/mL. The method is simple, accurate and sensitive, and it was applied to the determination of inosine in tablet samples with satisfactory results.

Key words: Inosine,spectrophotometric,Brilliant Cresyl blue